Postnatal Transplantation of Interneuronal Precursor Cells Decreases Anxiety-Like Behavior in Adult Mice
Postnatal Transplantation of Interneuronal Precursor Cells Decreases Anxiety-Like Behavior in Adult Mice
复制标题
产后神经元前体细胞移植可减少成年小鼠的焦虑样行为
DOI：
10.3727/096368912x657422
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发表时间：
2013
影响因子：
3.3
通讯作者：
Beatriz M. Longo
中科院分区：
文献类型：
--
作者：
M. F. Valente;S. Romariz;M. E. Calcagnotto;L. Ruiz;Luiz E. Mello;Roberto Frussa;Beatriz M. Longo
GABA能系统在调节焦虑水平中起重要作用，GABA能神经传递功能障碍似乎与广泛性焦虑障碍的发展有关。来自内侧神经节隆起（MGE）的前体细胞移植到小鼠脑内后具有迁移和分化为抑制性GABA能中间神经元的能力。因此，将来自MGE的神经元间前体细胞移植到出生后的大脑中可以改变神经元回路，增加GABA能张力并减少动物的焦虑样行为。我们的目的是通过评估小鼠的焦虑样行为来验证移植的MGE细胞的体内效应。将来自14天绿色荧光蛋白（GFP）胚胎的MGE细胞移植到新生小鼠中。在移植后15、30和60天，用高架十字迷宫（elevated plus maze，EMAZ）测试测试动物的焦虑行为。我们的研究结果表明，从MGE移植的细胞能够迁移到脑实质的不同区域，并分化为抑制性中间神经元。神经元前体细胞移植的动物焦虑水平降低，表明这些细胞在体内具有特定功能。我们认为，MGE源性神经元前体细胞移植到新生小鼠脑中可以增强与焦虑样行为相关的GABA能神经元回路的抑制功能。
The GABAergic system is critically involved in the modulation of anxiety levels, and dysfunction of GABAergic neurotransmission appears to be involved in the development of generalized anxiety disorder. Precursor cells from the medial ganglionic eminence (MGE) have the ability to migrate and differentiate into inhibitory GABAergic interneurons after being transplanted into the mouse brain. Thus, transplantation of interneuronal precursor cells derived from the MGE into a postnatal brain could modify the neuronal circuitry, increasing GABAergic tone and decreasing anxiety-like behavior in animals. Our aim was to verify the in vivo effects of transplanted MGE cells by evaluating anxiety-like behavior in mice. MGE cells from 14-day green fluorescent protein (GFP) embryos were transplanted into newborn mice. At 15, 30, and 60 days posttransplant, the animals were tested for anxiety behavior with the elevated plus maze (EPM) test. Our results show that transplanted cells from MGE were able to migrate to different regions of the brain parenchyma and to differentiate into inhibitory interneurons. The neuronal precursor cell transplanted animals had decreased levels of anxiety, indicating a specific function of these cells in vivo. We suggested that transplantation of MGE-derived neuronal precursors into neonate brain could strengthen the inhibitory function of the GABAergic neuronal circuitry related to anxiety-like behavior in mice.
影响因子：
4.6
作者：
H. Wichterle;D. Turnbull;S. Nery;G. Fishell;A. Álvarez-Buylla
通讯作者：
H. Wichterle;D. Turnbull;S. Nery;G. Fishell;A. Álvarez-Buylla
胚胎MGE前体细胞接枝到成年大鼠纹状体中，并改善6- OHDA的大鼠的运动症状。
影响因子：
23.9
作者：
Martínez-Cerdeño V;Noctor SC;Espinosa A;Ariza J;Parker P;Orasji S;Daadi MM;Bankiewicz K;Alvarez-Buylla A;Kriegstein AR
通讯作者：
Kriegstein AR
影响因子：
13.8
作者：
Holmes A;Quirk GJ
通讯作者：
Quirk GJ