LIPOPHAGY: a novel form of steroidogenic activity within the LEYDIG cell during the reproductive cycle of turtle
LIPOPHAGY: a novel form of steroidogenic activity within the LEYDIG cell during the reproductive cycle of turtle
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LIPOPHAGY：海龟生殖周期中 LEYDIG 细胞内类固醇生成活性的一种新形式
DOI：
10.1186/s12958-019-0462-2
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发表时间：
2019-02
影响因子：
4.4
通讯作者：
Chen Qiusheng
中科院分区：
文献类型：
--
作者：
Tarique Imran;Vistro Waseem Ali;Bai Xuebing;Yang Ping;Hong Chen;Huang Yufei;Haseeb Abdul;Liu Enxue;G;ahi Noor Samad;Xu Mengdi;Liu Yifei;Chen Qiusheng
研究背景睾酮合成是睾丸间质细胞（Leydig cell，LC）利用脂滴（lipid drops，LD）维持正常睾酮合成的一个重要过程，与精子发生间接相关。LD动员的调节，称为脂噬，在LC中仍然是很大程度上未知的。方法在本研究中，研究了中国鳖LC的类固醇生成活性和脂噬在一年的生殖周期，通过光学显微镜，免疫组织化学（IHC），免疫荧光（IF），结果LC在冬眠过程中表现出动态的甾体激素合成功能，3-HSD、波形蛋白和管状ER活性增强。在生殖阶段，LC中的小管泡状ER对3-HSD有弱的免疫阳性反应，表明具有持续的类固醇生成活性。ORO染色和透射电镜观察表明，冬眠期LC中LD的积累量大于繁殖期。这些LD与线粒体和溶酶体密切相关，动态地被中间丝包围，以促进LD的利用。溶酶体被发现直接附着在大的LD上，形成自噬管并吞噬LD，这表明在冬眠期间发生了微脂肪吞噬。此外，ATG 7的IHC（自噬相关基因7）和LC 3的IF（微管相关蛋白轻链3），p62（Sequestosome-1（SQSTM 1）和LAMP 1）Lysosomal-associated membrane protein 1）的结果显示了强表达，TEM进一步证实了自噬体和自溶酶体的存在以及它们在冬眠季节的融合。本研究提供了明确的证据表明，在冬眠期间，LD在LC中通过噬脂、溶酶体和线粒体消耗，这是中华鳖类固醇合成的一个关键方面。
BackgroundSteroidogenesis is an indispensable process that is indirectly associated with spermatogenesis in the Leydig cell (LC) to utilize the lipid droplets (LDs) that are critical to maintaining normal testosterone synthesis. The regulation of LD mobilization, known as lipophagy, in the LC is still largely unknown.MethodIn the present study, the LC of the Chinese soft-shelled turtle was investigated to identify the steroidogenic activity and lipophagy during the annual reproductive cycle by light microscopy, immunohistochemistry (IHC), immunofluorescence (IF), and transmission electron microscopy (TEM).ResultsThe LC showed a dynamic steroidogenic function with strong activity of 3-HSD, vimentin and tubular ER during hibernation by IHC and TEM. The tubulo-vesicular ER had a weak immunopositive reaction for 3-HSD in the LC during reproductive phase, suggesting persistent steroidogenic activity. ORO staining and TEM demonstrated that a larger number of LDs had accumulated in the LC during hibernation than in the reproductive phase. These LDs existed in close association with mitochondria and lysosomes by being dynamically surrounded by intermediate filaments to facilitate LD utilization. Lysosomes were found directly attached to large LDs, forming an autophagic tube and engulfing LDs, suggesting that micro-lipophagy occurs during hibernation. Furthermore, the IHC of ATG7 (Autophagy Related Gene 7) and the IF of the LC3 (Microtubule-associated protein light chain 3), p62 (Sequestosome-1 (SQSTM1) and LAMP1(Lysosomal-associated membrane protein 1) results demonstrated strong expression, and further confirmation by TEM showed the existence of an autophagosome and an autolysosome and their fusion during the hibernation season.ConclusionIn conclusion, the present study provides clear evidence of LD consumption in the LC by lipophagy, lysosome and mitochondria during the hibernation period, which is a key aspect of steroidogenesis in the Chinese soft-shelled turtle.
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影响因子：
11.1
作者：
He C;Klionsky DJ
通讯作者：
Klionsky DJ
DOI：
10.1016/b978-0-12-571139-5.50019-7
发表时间：
1983
期刊：
Recent progress in hormone research
影响因子：
--
作者：
L. Ewing;B. Zirkin
通讯作者：
L. Ewing;B. Zirkin
影响因子：
2.7
作者：
I. Mahmoud;R. Cyrus;T. Bennett;M. Woller;D. Montag
通讯作者：
I. Mahmoud;R. Cyrus;T. Bennett;M. Woller;D. Montag
影响因子：
2
作者：
B. Lofts;H. Tsui
通讯作者：
B. Lofts;H. Tsui
DOI：
10.1002/ar.1092420202
发表时间：
1995-06
期刊：
The Anatomical Record
影响因子：
--
作者：
J. Yi;Xue-ming Tang
通讯作者：
J. Yi;Xue-ming Tang