Dramatic differences in the roles in lipid metabolism of two isoforms of diacylglycerol kinase.
Dramatic differences in the roles in lipid metabolism of two isoforms of diacylglycerol kinase.
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DOI：
10.1021/bi800492c
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发表时间：
2008-09-09
期刊：
影响因子：
2.9
通讯作者：
Epand, Richard M.
中科院分区：
文献类型：
--
作者：
Milne, Stephen B.;Ivanova, Pavlina T.;Armstrong, Michelle D.;Myers, David S.;Lubarda, Jovana;Shulga, Yulia V.;Topham, Matthew K.;Brown, H. Alex;Epand, Richard M.
使用直接输注质量测定来自野生型或二酰甘油激酶-ε (DGKε) 或二酰甘油激酶-α (DGKα) 敲除小鼠的 SV40 转化成纤维细胞的甘油磷脂、多聚磷脂酰肌醇 (GPInsPn) 和二酰甘油 (DAG) 的脂质种类变化 光谱测定法。在野生型和 DGKε 敲除细胞之间，观察到多类甘油磷脂和多磷脂酰肌醇的含花生四烯酸（20:4 脂肪酸）脂质的显着差异。然而，在 DGKε 敲除细胞和野生型细胞之间，未观察到 DAG 的量或酰基链组成存在差异，这表明 DGKε 催化这些细胞中一小部分 DAG 的磷酸化。两种细胞系之间的花生四烯酸含量差异对于 GPInsPn 脂质而言最大，而对于 DAG 而言差异最小。这些发现表明，DGKε 在决定 20:4 GPInsPn 的富集方面发挥着重要作用，并且存在花生四烯酰磷脂酸从质膜选择性易位至内质网的途径。相反，在野生型小鼠或 DGKα 敲除小鼠的成纤维细胞的脂质提取物之间，任何类别的甘油磷脂或二酰基甘油的酰基链组成均未观察到显着差异。然而，来自 DGKα 敲除小鼠的细胞具有较高浓度的 DAG，这与 DGKα 对 DAG 的主要部分缺乏下调一致，而 DGKε 主要负责富集具有花生四烯酰酰基链的 GPInsPn。
Lipid species change for SV40 transformed fibroblasts from wild-type or from diacylglycerol kinase-ε (DGKε) or diacylglycerol kinase-α (DGKα) knock out mice, were determined for glycerophospholipids, poly-phosphatidylinositides (GPInsPn), and diacylglycerol (DAG) using direct infusion mass spectrometry. Dramatic differences in arachidonate (20:4 fatty acid)-containing lipids were observed for multiple classes of glycerophospholipids and polyphosphatidylinositides between wild-type and DGKε knock out cells. However, no difference was observed in either the amount or the acyl chain composition of DAG between DGKε knock out and wild-type cells, suggesting that DGKε catalyzed the phosphorylation of a minor fraction of the DAG in these cells. The differences in arachidonate content between the two cell lines were greatest for the GPInsPn lipids and least for DAG. These findings indicate that DGKε plays a significant role in determining the enrichment of GPInsPn with 20:4 and that there is a pathway for the selective translocation of arachidonoyl-phosphatidic acid from the plasma membrane to the endoplasmic reticulum. In contrast, no substantial difference was observed in the acyl chain composition of any class of glycerophospholipid or diacylglycerol between lipid extracts from fibroblasts from wild-type mice or from DGKα knock out mice. However, the cells from the DGKα knock out mice had a higher concentration of DAG, consistent with the lack of down regulation of the major fraction of DAG by DGKα, in contrast with DGKε that is primarily responsible for enrichment of GPInsPn with arachidonoyl acyl chains.
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DOI：
10.1046/j.1432-1327.2001.02116.x
发表时间：
2001-04-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Pichler, H;Gaigg, B;Daum, G
通讯作者：
Daum, G
影响因子：
4.8
作者：
Merino, Ernesto;Sanjuan, Miguel A.;Merida, Isabel
通讯作者：
Merida, Isabel
影响因子：
4.8
作者：
Milne, Stephen;Ivanova, Pavlina;Brown, H. Alex
通讯作者：
Brown, H. Alex
影响因子：
30.5
作者：
Olenchock, Benjamin A.;Guo, Rishu;Zhong, Xiao-Ping
通讯作者：
Zhong, Xiao-Ping
DOI：
10.1083/jcb.153.1.207
发表时间：
2001-04-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Sanjuán MA;Jones DR;Izquierdo M;Mérida I
通讯作者：
Mérida I