Investigations of intrinsic abnormalities in DNA-specific B lymphocytes from autoimmune mice.
Investigations of intrinsic abnormalities in DNA-specific B lymphocytes from autoimmune mice.
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自身免疫小鼠 DNA 特异性 B 淋巴细胞内在异常的研究。
DOI：
10.1016/0896-8411(89)90269-2
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发表时间：
1989
影响因子：
12.8
通讯作者：
Aldo-Benson,M
中科院分区：
文献类型：
--
作者：
Aldo-Benson,M
在系统性红斑狼疮的鼠模型和许多SLE患者中，抗天然DNA（dsDNA）抗体是疾病发病机制的主要贡献者。对DNA抗原自身耐受性的丧失可能与B细胞缺陷或调节细胞功能障碍有关。我们已经开发了对抗原DNA具有特异性的B细胞系，来自自身免疫性BWF 1小鼠品系和来自非自身免疫性BALB c品系，用于研究自身免疫中的固有B细胞缺陷。6株BWF 1细胞系和5株BALB c细胞系均不含Thy 1。2+细胞和酯酶阳性细胞，并且具有35 - 89%的与dsDNA-SRBC靶的玫瑰花结，已经在体外繁殖了24-36个月。这些细胞是非恶性的，生长因子依赖性的，并且在生长培养基中没有抗原或有丝分裂原。在细胞系中发现Lyt-1阳性细胞，但也存在Lyt-1阴性细胞。当EL-4上清液存在于培养物中时，它们对抗原DNA-HRBC作出反应，并且两种菌株的空斑形成细胞（PFC）反应的峰值相同。当来自两种菌株的细胞与不同量的T细胞因子一起培养时，自发抗体形成细胞（AFC）形成或对BWF 1和BALB c菌株之间的抗mu刺激的应答没有差异。在该培养系统中，BALB c脾细胞对DNA-HRBC不应答，但BWF 1脾细胞以及来自两个品系的细胞系细胞对该抗原应答。来自无应答的BALB c脾和应答的BWF 1脾的T细胞能够抑制来自两种菌株的细胞系B细胞对DNA-HRBC的免疫应答。在DNA存在下培养B细胞系2周可刺激BWF 1细胞系细胞，但抑制BALB c细胞系对抗原的反应。
In murine models of systemic lupus erythematosus and in many humans with SLE, antibodies against native DNA (dsDNA) are a major contributor to the pathogenesis of the disease. Loss of self-tolerance to the DNA antigen may be associated with B-cell defects or regulatory cell dysfunction. We have developed B-cell lines with specificity for the antigen DNA, from both the autoimmune BWF 1 mouse strain and from the non-autoimmune BALB c strain, to use in the investigation of inherent B-cell defects in autoimmunity. Six BWF 1 cell lines and five BALB c cell lines which are free of Thy1. 2+ cells and esterase positive cells, and have between 35 and 89% rosetting with dsDNA-SRBC targets, have been propagated in vitro for 24–36 months. The cells are non-malignant, growth-factor dependent and have no antigen or mitogen in the growth medium. Lyt-1 positive cells are found in the cell lines, but Lyt-1 negative cells are also present. They respond to the antigen DNA-HRBC when EL-4 supernatant is present in culture, and the peak of the plaque-forming cell (PFC) response is the same for both strains. When cells from both strains are cultured with varying amounts of T-cell factors, there is no difference in spontaneous antibody-forming cell (AFC) formation or in response to anti-mu stimulation between BWF 1 and BALB c strains. BALB c spleen cells do not respond to DNA-HRBC in this culture system, but BWF 1 spleen cells, as well as cell line cells from both strains, respond to this antigen. T cells from non-responding BALB c spleen and responding BWF 1 spleen are able to suppress the immune response to DNA-HRBC of cell line B cells from both strains. Propagating B-cell lines in the presence of DNA for 2 weeks stimulates BWF 1 cell line cells, but suppresses the response of BALB c cell lines to antigen.
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持续生长的抗原特异性 B 细胞系克隆无反应的体外模型。
影响因子：
4.3
作者：
Aldo-Benson,MA
通讯作者：
Aldo-Benson,MA
NZB/W 小鼠肾小球肾炎的发病机制
影响因子：
15.3
作者：
P. H. Lambert;Frank J. Dixon
通讯作者：
Frank J. Dixon
NZB T 淋巴细胞在体外抗 DNA 抗体产生中的调节作用。
影响因子：
4.4
作者：
C. Laskin;G. Haddad;C. Solonínka
通讯作者：
C. Solonínka
免疫学自我识别机制研究.
影响因子：
4.4
作者：
Edward L. Triplett
通讯作者：
Edward L. Triplett
自身抗体的细胞起源——一个令人费解的问题。
DOI：
10.1016/0002-9343(76)90364-8
发表时间：
1976
期刊：
The American journal of medicine
影响因子：
--
作者：
A. Bankhurst;R. Williams
通讯作者：
R. Williams