Feasibility of whole-body functional mouse imaging using helical pinhole SPECT.
Feasibility of whole-body functional mouse imaging using helical pinhole SPECT.
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DOI：
10.1007/s11307-009-0234-z
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发表时间：
2010-01
影响因子：
3.1
通讯作者：
Chin, Bennett B.
中科院分区：
文献类型：
--
作者：
Metzler, Scott D.;Vemulapalli, Sreekanth;Jaszczak, Ronald J.;Akabani, Gamal;Chin, Bennett B.
放射性标记示踪剂的详细体内全身生物分布可以表征疾病的纵向进展，并随治疗干预而改变。由于具有广泛的遗传特征和手术创建的疾病模型，小鼠的小动物成像特别有吸引力。使用针孔准直的单光子发射计算机断层扫描（SPECT）成像提供了高分辨率和灵敏度，但使用圆形采集的传统方法导致严重的图像截断和数据采样不完整，从而阻止了对全身放射性示踪剂生物分布的准确测定。本研究描述了缓解这些影响的螺旋采集路径的可行性。针孔孔径的螺旋路径是通过与扫描仪旋转轴（AOR）对齐的外部机器人平台实现的。幻影和鼠标扫描采用螺旋路径和圆形或双圆形轨道在相同的旋转半径（ROR）进行。双圆轨道由两个360度扫描组成，通过轴向位移分开，以增加轴向视野（FOV）并改善完整采样特性。用螺旋路径重建的幻影和小鼠显示出良好的图像质量，并且在视觉上没有截断和轴向模糊伪影。圆形轨道产生了具有伪影和有限有效视场的重建。双圆扫描扩大了轴向视场，但仍然存在截断和采样伪影。螺旋路径可以提供完整的采样数据和大的有效视场，在获得三维全身生物体内分布的同时，仍然保持孔径到物体的小距离，具有良好的灵敏度和分辨率。
Detailed in vivo whole-body biodistributions of radiolabeled tracers may characterize the longitudinal progression of disease, and changes with therapeutic interventions. Small-animal imaging in mice is particularly attractive due to the wide array of well characterized genetically and surgically created models of disease. Single Photon Emission Computed Tomography (SPECT) imaging using pinhole collimation provides high resolution and sensitivity, but conventional methods using circular acquisitions result in severe image truncation and incomplete sampling of data which prevent the accurate determination of whole-body radiotracer biodistributions. This study describes the feasibility of helical acquisition paths to mitigate these effects. Helical paths of pinhole apertures were implemented using an external robotic stage aligned with the axis of rotation (AOR) of the scanner. Phantom and mouse scans were performed using helical paths and either circular or bi-circular orbits at the same radius of rotation (ROR). The bi-circular orbits consisted of two 360-degree scans separated by an axial shift to increase the axial field of view (FOV) and to improve the complete-sampling properties. Reconstructions of phantoms and mice acquired with helical paths show good image quality and are visually free of both truncation and axial-blurring artifacts. Circular orbits yielded reconstructions with both artifacts and a limited effective FOV. The bi-circular scans enlarged the axial FOV, but still suffered from truncation and sampling artifacts. Helical paths can provide complete sampling data and large effective FOV, yielding 3D full-body in vivo biodistributions while still maintaining a small distance from the aperture to the object for good sensitivity and resolution.
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DOI：
10.1007/s00259-002-0805-6
发表时间：
2002-07-01
影响因子：
9.1
作者：
Beekman, FJ;McElroy, DP;Cherry, SR
通讯作者：
Cherry, SR
影响因子：
1.8
作者：
Metzler, SD;Jaszczak, RJ
通讯作者：
Jaszczak, RJ
影响因子：
3.5
作者：
DiFilippo, Frank P.
通讯作者：
DiFilippo, Frank P.
影响因子：
1.8
作者：
Metzler, SD;Greer, KL;Jaszczak, RJ
通讯作者：
Jaszczak, RJ
影响因子：
1.8
作者：
Metzler, SD;Bowsher, JE;Jaszczak, RJ
通讯作者：
Jaszczak, RJ