Neurotransmitter phenotype and axonal projection patterns of VIP-expressing neurons in the inferior colliculus.
Neurotransmitter phenotype and axonal projection patterns of VIP-expressing neurons in the inferior colliculus.
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DOI：
10.1016/j.jchemneu.2022.102189
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发表时间：
2022-12
影响因子：
2.8
通讯作者：
Schofield, Brett R.
中科院分区：
文献类型：
--
作者：
Beebe, Nichole L.;Silveira, Marina A.;Goyer, David;Noftz, William A.;Roberts, Michael T.;Schofield, Brett R.
下丘（IC）中的神经元是中央听觉通路的中脑枢纽，向其他听觉脑区域发送上升和下降的投射，以及向其他感觉和非感觉脑区域的投射。然而，各类 IC 神经元的轴突投射模式仍然很大程度上未知。血管活性肠多肽（VIP）是一种神经肽，由许多大脑区域的神经元亚群表达。我们最近发现了一类 IC 星状神经元，我们称之为 VIP 神经元，因为它们在 VIP-IRES-Cre × Ai14 小鼠中被 tdTomato (tdT) 表达标记。在这里，利用荧光原位杂交，我们发现 VIP-IRES-Cre × Ai14 小鼠中的 tdT+ 神经元表达 Vglut2（谷氨酸能神经元的标记物）和 VIP，表明 VIP 神经元使用谷氨酸能和 VIPergic 信号传导来影响其突触后靶标。接下来，使用具有 Cre 依赖性 eGFP 构建体的病毒转染，我们标记了 VIP 神经元的轴突投影。作为一个群体，VIP 神经元在同侧和对侧 IC 内进行内在投射，并在 IC 的所有主要目标外进行投射。在听觉系统内，VIP神经元发送轴突并在更高的中心形成轴突，包括内侧膝状体核和IC臂核。密度较低的投射终止于下部中心，包括外侧丘系核、上橄榄复合体和背侧耳蜗核。 VIP 神经元还投射到几个非听觉脑区，包括上丘、导水管周围灰质和楔形核。与 VIP 神经元内在特性的同质性相比，VIP 投射的多样性表明 VIP 神经元在微电路水平上发挥保守作用，可能涉及通过谷氨酸能和 VIPergic 信号传导的神经调节，但通过参与不同的投射途径在系统水平上支持多种功能。下丘中的一群星状神经元表达谷氨酸和 VIP。这些神经元投射到脑干和丘脑中的广泛目标（绿色阴影），表明它们在多种行为中发挥作用。
Neurons in the inferior colliculus (IC), the midbrain hub of the central auditory pathway, send ascending and descending projections to other auditory brain regions, as well as projections to other sensory and non-sensory brain regions. However, the axonal projection patterns of individual classes of IC neurons remain largely unknown. Vasoactive intestinal polypeptide (VIP) is a neuropeptide expressed by subsets of neurons in many brain regions. We recently identified a class of IC stellate neurons that we called VIP neurons because they are labeled by tdTomato (tdT) expression in VIP-IRES-Cre × Ai14 mice. Here, using fluorescence in situ hybridization, we found that tdT+ neurons in VIP-IRES-Cre × Ai14 mice express Vglut2, a marker of glutamatergic neurons, and VIP, suggesting that VIP neurons use both glutamatergic and VIPergic signaling to influence their postsynaptic targets. Next, using viral transfections with a Cre-dependent eGFP construct, we labeled the axonal projections of VIP neurons. As a group, VIP neurons project intrinsically, within the ipsilateral and contralateral IC, and extrinsically to all the major targets of the IC. Within the auditory system, VIP neurons sent axons and formed axonal boutons in higher centers, including the medial geniculate nucleus and the nucleus of the brachium of the IC. Less dense projections terminated in lower centers, including the nuclei of the lateral lemniscus, superior olivary complex, and dorsal cochlear nucleus. VIP neurons also project to several non-auditory brain regions, including the superior colliculus, periaqueductal gray, and cuneiform nucleus. The diversity of VIP projections compared to the homogeneity of VIP neuron intrinsic properties suggests that VIP neurons play a conserved role at the microcircuit level, likely involving neuromodulation through glutamatergic and VIPergic signaling, but support diverse functions at the systems level through their participation in different projection pathways. A population of stellate neurons in the inferior colliculus express glutamate and VIP. These neurons project to a wide range of targets (shaded green) in the brainstem and thalamus, suggesting a role in a diverse range of behaviors.
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