In Vivo Therapeutic Silencing of Hypoxia-Inducible Factor 1 Alpha (HIF-1α) Using Single-Walled Carbon Nanotubes Noncovalently Coated with siRNA.
In Vivo Therapeutic Silencing of Hypoxia-Inducible Factor 1 Alpha (HIF-1α) Using Single-Walled Carbon Nanotubes Noncovalently Coated with siRNA.
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利用非共价包被小干扰RNA（siRNA）的单壁碳纳米管在体内对缺氧诱导因子1α（HIF - 1α）进行治疗性沉默 。
DOI：
10.1007/s12274-009-9026-7
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发表时间：
2009-04-17
期刊：
影响因子：
9.9
通讯作者：
Powis, Garth
中科院分区：
文献类型：
--
作者：
Bartholomeusz, Geoffrey;Cherukuri, Paul;Kingston, John;Cognet, Laurent;Lemos, Robert, Jr.;Leeuw, Tonya K.;Gumbiner-Russo, Laura;Weisman, R. Bruce;Powis, Garth
本文描述了一种通过将未修饰的小干扰RNA（siRNA）与原始单壁碳纳米管（SWCNT）非共价复合，从而将siRNA递送至癌细胞的新方法。这些复合物是通过在siRNA溶液中对原始SWCNT进行简单超声处理制备的，在此过程中，siRNA既作为“货物”，又作为SWCNT的悬浮剂。当将含有靶向缺氧诱导因子1α（HIF - 1α）的siRNA的复合物添加到在含血清培养基中生长的细胞中时，细胞内HIF - 1α的活性受到了强烈的特异性抑制。在多种癌细胞类型中都观察到了对SWCNT/siRNA复合物产生生物学反应的能力。此外，在携带MiaPaCa - 2/HRE肿瘤的小鼠中，瘤内给予SWCNT - HIF - 1α siRNA复合物显著抑制了肿瘤中HIF - 1α的活性。由于在许多人类癌症中都发现HIF - 1α水平升高，并且与治疗抗性和患者生存率降低有关，这些结果表明SWCNT/siRNA复合物可能具有作为治疗剂的价值。
A new approach is described for delivering small interfering RNA (siRNA) into cancer cells by noncovalently complexing unmodified siRNA with pristine single-walled carbon nanotubes (SWCNTs). The complexes were prepared by simple sonication of pristine SWCNTs in a solution of siRNA, which then served both as the cargo and as the suspending agent for the SWCNTs. When complexes containing siRNA targeted to hypoxia-inducible factor 1 alpha (HIF-1α) were added to cells growing in serum containing culture media, there was strong specific inhibition of cellular HIF-1α activity. The ability to obtain a biological response to SWCNT/siRNA complexes was seen in a wide variety of cancer cell types. Moreover, intratumoral administration of SWCNT-HIF-1α siRNA complexes in mice bearing MiaPaCa-2/HRE tumors significantly inhibited the activity of tumor HIF-1α. As elevated levels of HIF-1α are found in many human cancers and are associated with resistance to therapy and decreased patient survival, these results imply that SWCNT/siRNA complexes may have value as therapeutic agents.
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影响因子：
15
作者：
Kam, NWS;Dai, HJ
通讯作者：
Dai, HJ
影响因子：
2.8
作者：
Nikolaev, P;Bronikowski, MJ;Smalley, RE
通讯作者：
Smalley, RE
DOI：
10.1073/pnas.0707654105
发表时间：
2008-02-05
影响因子：
11.1
作者：
Liu, Zhuang;Davis, Corrine;Dai, Hongjie
通讯作者：
Dai, Hongjie
影响因子：
11.2
作者：
Liao, Debbie;Corle, Courtney;Johnson, Randall S.
通讯作者：
Johnson, Randall S.
DOI：
10.1016/j.nano.2005.03.003
发表时间：
2005-06-01
影响因子：
5.4
作者：
Meng, Jie;Song, Li;Xie, Sishen
通讯作者：
Xie, Sishen