Growth and site-specific organization of micron-scale biomolecular devices on living mammalian cells.
Growth and site-specific organization of micron-scale biomolecular devices on living mammalian cells.
复制标题
微尺度生物分子装置在哺乳动物细胞上的生长和位点特异性组织。
DOI：
10.1038/s41467-021-25890-z
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发表时间：
2021-09-30
影响因子：
16.6
通讯作者：
Schulman R
中科院分区：
文献类型：
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作者：
Jia S;Phua SC;Nihongaki Y;Li Y;Pacella M;Li Y;Mohammed AM;Sun S;Inoue T;Schulman R
Mesoscale molecular assemblies on the cell surface, such as cilia and filopodia, integrate information, control transport and amplify signals. Designer cell-surface assemblies could control these cellular functions. Such assemblies could be constructed from synthetic components ex vivo, making it possible to form such structures using modern nanoscale self-assembly and fabrication techniques, and then oriented on the cell surface. Here we integrate synthetic devices, micron-scale DNA nanotubes, with mammalian cells by anchoring them by their ends to specific cell surface receptors. These filaments can measure shear stresses between 0-2 dyn/cm2, a regime important for cell signaling. Nanotubes can also grow while anchored to cells, thus acting as dynamic cell components. This approach to cell surface engineering, in which synthetic biomolecular assemblies are organized with existing cellular architecture, could make it possible to build new types of sensors, machines and scaffolds that can interface with, control and measure properties of cells. Mesoscale molecular assemblies on the cell surface integrate information and amplify signals. Here the authors integrate DNA nanotubes in a controlled manner with mammalian cells to act as sheer stress meters.
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影响因子：
38.3
作者：
Hariadi RF;Sommese RF;Adhikari AS;Taylor RE;Sutton S;Spudich JA;Sivaramakrishnan S
通讯作者：
Sivaramakrishnan S
影响因子：
38.3
作者：
Jiang, Wen;Kim, Betty Y. S.;Chan, Warren C. W.
通讯作者：
Chan, Warren C. W.
影响因子：
17.1
作者：
Agrawal, Deepak K.;Jiang, Ruoyu;Schulman, Rebecca
通讯作者：
Schulman, Rebecca
使用工程细胞折纸簇对细胞间通信进行编程
影响因子：
15
作者：
Ge, Zhilei;Liu, Jiangbo;Fan, Chunhai
通讯作者：
Fan, Chunhai
影响因子：
64.8
作者：
通讯作者：
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