Hypophysiotropic Role and Hypothalamic Gene Expression of Corticotropin‐Releasing Factor and Vasopressin in Rats Injected with Interleukin‐1β Systemically or into the Brain Ventricles
Hypophysiotropic Role and Hypothalamic Gene Expression of Corticotropin‐Releasing Factor and Vasopressin in Rats Injected with Interleukin‐1β Systemically or into the Brain Ventricles
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全身注射白介素-1β或脑室注射促肾上腺皮质激素释放因子和加压素的促垂体作用和下丘脑基因表达
DOI：
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发表时间：
1994
影响因子：
3.2
通讯作者：
C. Rivier
中科院分区：
文献类型：
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作者：
S. Lee;C. Rivier
将完整成年雄性大鼠静脉注射（i.v., 400 ng/kg）、腹腔注射（i.v., 400 ng/kg）或脑室内注射（i.c.v., 100 ng/kg）白细胞介素- 1β (IL - 1β)或其载体。与小鼠相比，IL - 1β诱导30分钟后血浆ACTH水平显著升高（P<0.01），与细胞因子给药方式无关。这些变化在给予针对促肾上腺皮质激素释放因子（CRF）的特异性抗体的大鼠中明显减弱。相比之下，抗利尿激素（VP）抗体显著减弱了体外注射IL - 1β释放的ACTH，但仅轻微改变了全身注射细胞因子的效果。然后，我们使用半定量原位杂交分析来测量稳态mRNA水平的变化，因为它们可能发生在相同剂量的IL - 1β的反应中。给药后，下丘脑室旁（PVN）部分的基因表达测量表明，CRF和VP的杂交信号均可测量到。注射IL - 1β 5 h后，大鼠的CRF和VP基因表达均未见明显变化。与此相反，体外注射细胞因子显著（P<0.01）提高了5 h后测定的CRF和VP mRNA水平。
Intact adult male rats were injected intravenously (i.v., 400 ng/kg), intraperitoneally (i.p., 400 ng/kg) or intracerebroventricularly (i.c.v., 100 ng/kg) with interleukin‐1β (IL‐1β) or its vehicle. In comparison with vehicle‐treated animals, IL‐1β induced significant (P<0.01) increases in plasma ACTH levels measured 30 min later regardless of the route of cytokine administration. These changes were markedly blunted in rats administered specific antibodies directed against corticotropin‐releasing factor (CRF). In contrast, vasopressin (VP) antibodies significantly blunted ACTH released by the i.c.v. injection of IL‐1β, but only modestly altered the effect of the systemic injection of the cytokine. We then used semi‐quantitative in situ hybridization analysis to measure changes in steady‐state mRNA levels, as they might occur in response to these same doses of IL‐1β. Following administration of the vehicle, measurement of gene expression in the paraventricular (PVN) portion of the hypothalamus indicated a measurable amount of hybridization signals for both CRF and VP. No detectable changes in either CRF or VP gene expression were observed in rats injected with IL‐1β i.v. or i.p. 5 h earlier. In contrast, the i.c.v. administration of the cytokine significantly (P<0.01) increased both CRF and VP mRNA levels measured 5 h later.
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使用针对合成肽片段产生的抗血清检测和纯化抑制素。
影响因子：
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作者：
Vaughan,JM;Rivier,J;Corrigan,AZ;McClintock,R;Campen,CA;Jolley,D;Voglmayr,JK;Bardin,CW;Rivier,C;Vale,W
通讯作者：
Vale,W
影响因子：
4.1
作者：
SWANSON, LW;SAWCHENKO, PE;VALE, WW
通讯作者：
VALE, WW
影响因子：
56.9
作者：
SAPOLSKY, R;RIVIER, C;VALE, W
通讯作者：
VALE, W
促肾上腺皮质激素对注入大鼠正中隆起的白介素-1β的反应取决于儿茶酚胺的局部释放。
影响因子：
4.8
作者：
Matta,S;Singh,J;Newton,R;Sharp,BM
通讯作者：
Sharp,BM
研究加压素和促肾上腺皮质激素释放因子之间相互作用对大鼠促肾上腺皮质激素释放的影响。
影响因子：
4.8
作者：
Rivier,C;Rivier,J;Mormede,P;Vale,W
通讯作者：
Vale,W