StemCellDB: the human pluripotent stem cell database at the National Institutes of Health.
StemCellDB: the human pluripotent stem cell database at the National Institutes of Health.
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DOI：
10.1016/j.scr.2012.09.002
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发表时间：
2013-01
影响因子：
1.2
通讯作者：
McKay, Ronald D. G.
中科院分区：
文献类型：
--
作者：
Mallon, Barbara S.;Chenoweth, Josh G.;Johnson, Kory R.;Hamilton, Rebecca S.;Tesar, Paul J.;Yavatkar, Amarendra S.;Tyson, Leonard J.;Park, Kyeyoon;Chen, Kevin G.;Fann, Yang C.;McKay, Ronald D. G.
诱导多能干细胞技术产生的兴奋很大程度上与疾病建模的可能性以及个性化细胞治疗的可能性有关。然而，要实现这一点，重要的是要了解“正常”的多能性状态，包括其内在的可变性。我们对21个hESC系和8个hPSC系进行了各种分子图谱分析，以生成多潜能干细胞未分化状态的全面快照。对基因表达数据的分析显示，与之前的报告一致，没有IPSC特有的基因表达模式。我们进一步比较了在2种培养基中分化为类胚体的细胞，以及20个成年组织。根据这一分析，我们已经生成了一份定义多能性的基因列表，并为多能性状态建立了一个基线。最后，我们提供了在两种分化条件下丰富的基因清单，这些基因显示出对独立细胞命运的拟议偏向。
Much of the excitement generated by induced pluripotent stem cell technology is concerned with the possibility of disease modeling as well as the potential for personalized cell therapy. However, to pursue this it is important to understand the ‘normal’ pluripotent state including its inherent variability. We have performed various molecular profiling assays for 21 hESC lines and 8 hiPSC lines to generate a comprehensive snapshot of the undifferentiated state of pluripotent stem cells. Analysis of the gene expression data revealed no iPSC-specific gene expression pattern in accordance with previous reports. We further compared cells, differentiated as embryoid bodies in 2 media proposed to initiate differentiation towards separate cell fates, as well as 20 adult tissues. From this analysis we have generated a gene list which defines pluripotency and establishes a baseline for the pluripotent state. Finally, we provide lists of genes enriched under both differentiation conditions which show the proposed bias toward independent cell fates.
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影响因子：
64.8
作者：
Park, In-Hyun;Zhao, Rui;Daley, George Q.
通讯作者：
Daley, George Q.
影响因子：
64.5
作者：
Takahashi, Kazutoshi;Yamanaka, Shinya
通讯作者：
Yamanaka, Shinya
影响因子：
56.9
作者：
Yu, Junying;Vodyanik, Maxim A.;Thomson, James A.
通讯作者：
Thomson, James A.
影响因子：
64.8
作者：
Tesar, Paul J.;Chenoweth, Josh G.;McKay, Ronald D. G.
通讯作者：
McKay, Ronald D. G.
影响因子：
64.5
作者：
Takahashi, Kazutoshi;Tanabe, Koji;Yamanaka, Shinya
通讯作者：
Yamanaka, Shinya