Disinfection and healing effects of 222-nm UVC light on methicillin-resistant Staphylococcus aureus infection in mouse wounds.
Disinfection and healing effects of 222-nm UVC light on methicillin-resistant Staphylococcus aureus infection in mouse wounds.
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222 nm UVC 光对小鼠伤口耐甲氧西林金黄色葡萄球菌感染的消毒和愈合作用。
DOI：
10.1016/j.jphotobiol.2017.10.030
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发表时间：
2018-01
期刊：
影响因子：
--
通讯作者：
Nakane A
中科院分区：
文献类型：
--
作者：
Narita K;Asano K;Morimoto Y;Igarashi T;Hamblin MR;Dai T;Nakane A
已知UVC辐射具有高度杀菌性。然而，暴露于254-nm-UVC光会导致人体细胞中的环丁烷嘧啶二聚体（CPD）等DNA损伤，并且在长期重复暴露后会诱发皮肤癌。据报道，短波UVC被细胞膜和细胞质中的蛋白质吸收，并且不能到达人体细胞的细胞核。因此，用222-nm UVC照射可能是对人体细胞有效消毒和生物安全性的最佳组合。在这项研究中，222-nm紫外线的生物有效性进行了研究，使用耐甲氧西林金黄色葡萄球菌（MRSA）感染的皮肤伤口的小鼠模型。与未照射皮肤相比，222 nm UVC照射显著减少了皮肤表面的细菌数量。在照射后第3、5、8和12天评估的伤口中的细菌计数表明，222 nm UVC的杀菌效果等于或优于254 nm UVC。组织学分析显示，角质形成细胞的迁移，这是必不可少的伤口愈合过程中受损的伤口照射与254 nm的UVC，但在222 nm的UVC照射的伤口不受影响。在222-nm UVC照射的伤口的表皮或真皮中未检测到CPD表达细胞，而在254-nm UVC照射的表皮和真皮中均发现CPD表达细胞。这些结果表明，222 nm的UVC光可能是一种安全有效的方法，以减少手术部位和其他伤口感染的发生率。
UVC radiation is known to be highly germicidal. However, exposure to 254-nm-UVC light causes DNA lesions such as cyclobutane pyrimidine dimers (CPD) in human cells, and can induce skin cancer after long-term repeated exposures. It has been reported that short wavelength UVC is absorbed by proteins in the membrane and cytosol, and fails to reach the nucleus of human cells. Hence, irradiation with 222-nm UVC might be an optimum combination of effective disinfection and biological safety to human cells. In this study, the biological effectiveness of 222-nm UVC was investigated using a mouse model of a skin wound infected with methicillin-resistant Staphylococcus aureus (MRSA). Irradiation with 222-nm UVC significantly reduced bacterial numbers on the skin surface compared with non-irradiated skin. Bacterial counts in wounds evaluated on days 3, 5, 8 and 12 after irradiation demonstrated that the bactericidal effect of 222-nm UVC was equal to or more effective than 254-nm UVC. Histological analysis revealed that migration of keratinocytes which is essential for the wound healing process was impaired in wounds irradiated with 254-nm UVC, but was unaffected in 222-nm UVC irradiated wounds. No CPD-expressing cells were detected in either epidermis or dermis of wounds irradiated with 222-nm UVC, whereas CPD-expressing cells were found in both epidermis and dermis irradiation with 254-nm UVC. These results suggest that 222-nm UVC light may be a safe and effective way to reduce the rate of surgical site and other wound infections.
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影响因子：
4.9
作者：
Dai, Tianhong;Garcia, Barbara;Hamblin, Michael R.
通讯作者：
Hamblin, Michael R.
影响因子：
3.7
作者：
Buonanno M;Randers-Pehrson G;Bigelow AW;Trivedi S;Lowy FD;Spotnitz HM;Hammer SM;Brenner DJ
通讯作者：
Brenner DJ
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
3.7
作者：
Dawes JM;Antunes-Martins A;Perkins JR;Paterson KJ;Sisignano M;Schmid R;Rust W;Hildebrandt T;Geisslinger G;Orengo C;Bennett DL;McMahon SB
通讯作者：
McMahon SB
影响因子：
3.1
作者：
NAKANE, A;OKAMOTO, M;MINAGAWA, T
通讯作者：
MINAGAWA, T