Cytoskeletal abnormalities in motor neuron disease. An immunocytochemical study.
Cytoskeletal abnormalities in motor neuron disease. An immunocytochemical study.
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运动神经元疾病中的细胞骨架异常。
DOI：
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Brian H. Anderton
中科院分区：
文献类型：
--
作者：
P. Leigh;A. Dodson;Michael Swash;J. Brion;Brian H. Anderton
用抗细胞骨架蛋白抗体的免疫细胞化学方法研究了肌萎缩侧索硬化型运动神经元病（MND）患者和正常脊髓的分子差异。识别磷酸化神经丝表位的单克隆抗体弥散标记正常和MND前角细胞的比例，但不允许正常和MND组织之间的分化。然而，在一些MND和控制前角细胞，密集的“絮状”积累标记的抗体识别磷酸化神经丝抗原决定簇。这些磷酸化神经丝的积累表明细胞骨架调节异常，但既不是MND的常见特征，也不是其特异性特征。轴突球状体，这是常见的在正常的MND组织，标记的所有抗神经丝抗体。正常出现的轴突，但不是球体，在MND和对照组织中被确定的抗肌动蛋白抗体，表明肌动蛋白可能是从细胞质域引起的球体缺席。总之，我们还没有发现特定的翻译后变化的细胞骨架蛋白在MND，特别是磷酸化的神经丝抗原决定簇是共同的MND和控制前角细胞。
Immunocytochemistry with antibodies against cytoskeletal proteins has been used to search for molecular differences in the spinal cord from patients with motor neuron disease (MND) of amyotrophic lateral sclerosis type and normal spinal cord. Monoclonal antibodies which recognize phosphorylated neurofilament epitopes diffusely labelled a proportion of normal and MND anterior horn cells, but did not permit differentiation between normal and MND tissue. However, in some MND and control anterior horn cells, dense 'floccular' accumulations were labelled by antibodies recognizing phosphorylated neurofilament epitopes. These accumulations of phosphorylated neurofilaments suggest abnormalities of cytoskeletal regulation, but were neither a common nor a specific feature of MND. Axonal spheroids, which were as common in normal as in MND tissue, were labelled by all antineurofilament antibodies. Normal-appearing axons, but not spheroids, in MND and control tissue were identified by an antiactin antibody, indicating that actin may be absent from the cytoplasmic domain which gives rise to spheroids. In summary, we have not found specific posttranslational changes of cytoskeletal proteins in MND and, in particular, phosphorylated neurofilament epitopes are common to both MND and control anterior horn cells.
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运动神经元的实验性神经毒性疾病：神经原纤维病理学。
影响因子：
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作者：
Griffin,J;Cork,LC;Troncoso,JC;Price,DL
通讯作者：
Price,DL
影响因子：
56.9
作者：
GOLDMAN, JE;YEN, SH;PERESS, NS
通讯作者：
PERESS, NS
DOI：
10.1073/pnas.83.11.4040
发表时间：
1986-06-01
影响因子：
11.1
作者：
WOOD, JG;MIRRA, SS;BINDER, LI
通讯作者：
BINDER, LI
DOI：
10.1073/pnas.83.13.4913
发表时间：
1986-07-01
影响因子：
11.1
作者：
GRUNDKEIQBAL, I;IQBAL, K;BINDER, LI
通讯作者：
BINDER, LI
运动神经元疾病患者和对照患者轴突肿胀中的磷酸依赖性和独立神经丝表位。
DOI：
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发表时间：
1987
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Schmidt,ML;Carden,MJ;Lee,VM;Trojanowski,JQ
通讯作者：
Trojanowski,JQ