Stage and tissue-specific prognostic impact of miR-182 in NSCLC.
Stage and tissue-specific prognostic impact of miR-182 in NSCLC.
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DOI：
10.1186/1471-2407-14-138
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发表时间：
2014-02-27
期刊：
影响因子：
3.8
通讯作者：
Bremnes RM
中科院分区：
文献类型：
--
作者：
Stenvold H;Donnem T;Andersen S;Al-Saad S;Busund LT;Bremnes RM
MicroRNA (miR)-182在癌症中经常上调，通常被视为一种致癌基因，可能与血管生成有关。我们旨在探讨miR-182在非小细胞肺癌（NSCLC）中的影响，并更明确地探讨其与血管生成标志物的相关性。从335例未选择的I至IIIA期非小细胞肺癌中，在组织微阵列块（tma）中收集了重复肿瘤和肿瘤相关基质核心。采用原位杂交（ISH）检测miR-182在肿瘤细胞中的表达，免疫组化（IHC）检测血管生成相关蛋白标志物的表达。在单因素分析中，miR-182的高肿瘤细胞表达是鳞状细胞癌（SCC, P = 0.042）和II期患者（P = 0.003）的积极预后因素。同样在多因素分析中，在同一组中，肿瘤细胞miR-182高表达与良好预后相关（SCC: HR 0.57, CI 95% 0.33-0.99, P = 0.048； II期：HR 0.50, CI 95% 0.28-0.90, P = 0.020）。我们发现miR-182与血管生成相关标志物FGF2、HIF2α和MMP-7之间存在显著相关性。在SCC患者和II期患者中，肿瘤细胞miR-182高表达是一个独立的阳性预后因素。
MicroRNA (miR)-182 is frequently upregulated in cancers, has generally been viewed as an oncogene and is possibly connected to angiogenesis. We aimed to explore what impact miR-182 has in non-small cell lung cancer (NSCLC), and more explicitly its correlation with angiogenic markers. From 335 unselected stage I to IIIA NSCLC carcinomas, duplicate tumor and tumor-associated stromal cores were collected in tissue microarray blocks (TMAs). In situ hybridization (ISH) was used to detect the expression of miR-182 in tumor cells, and immunohistochemistry (IHC) was used to detect the expression of angiogenesis related protein markers. In univariate analyses, high tumor cell expression of miR-182 was a positive prognostic factor for patients with squamous cell carcinoma (SCC, P = 0.042) and stage II patients (P = 0.003). Also in the multivariate analysis, high tumor cell miR-182 expression was associated with a good prognosis in the same groups (SCC: HR 0.57, CI 95% 0.33-0.99, P = 0.048; stage II: HR 0.50, CI 95% 0.28-0.90, P = 0.020). We found significant correlations between miR-182 and the angiogenesis related markers FGF2, HIF2α and MMP-7. In patients with SCC and in stage II patients, high tumor cell miR-182 expression is an independent positive prognostic factor.
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影响因子：
5.3
作者：
Andersen, Sigve;Eilertsen, Marte;Bremnes, Roy M.
通讯作者：
Bremnes, Roy M.
影响因子：
4.8
作者：
Jorgensen, Stine;Baker, Adam;Nielsen, Boye Schnack
通讯作者：
Nielsen, Boye Schnack
DOI：
10.1073/pnas.0808263106
发表时间：
2009-02-10
影响因子：
11.1
作者：
Segura, Miguel F.;Hanniford, Douglas;Hernando, Eva
通讯作者：
Hernando, Eva
影响因子：
2.8
作者：
Barshack, Iris;Lithwick-Yanai, Gila;Perelman, Marina
通讯作者：
Perelman, Marina
miR-182 作为神经胶质瘤进展和患者生存的预后标志物
影响因子：
6
作者：
Jiang, Lili;Mao, Pu;Li, Jun
通讯作者：
Li, Jun