Inhibition of steroid-induced prostatic hyperplasia in rats by treatment with anti-androgen (TZP-4238).
Inhibition of steroid-induced prostatic hyperplasia in rats by treatment with anti-androgen (TZP-4238).
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通过抗雄激素 (TZP-4238) 治疗抑制大鼠类固醇诱导的前列腺增生。
DOI：
10.1507/endocrj.40.479
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发表时间：
1993
影响因子：
2
通讯作者：
K. Watanabe
中科院分区：
文献类型：
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作者：
M. Murakoshi;M. Tagawa;R. Inada;M. Suzuki;A. Mizokami;K. Watanabe
研究了合成的甾体抗雄激素TZP-4238对甾体诱导的大鼠前列腺增生的作用。雄性Wistar大鼠分为4个实验组。第1组为完整对照组。其他动物被阉割。去势动物用1）睾酮1 mg/头加17 β-雌二醇（E2）0.01 mg/头（组2）、2）睾酮加E2 + TZP-4238 8 mg/kg（组3）和3）睾酮加E2 +醋酸氯地孕酮（CMA）20 mg/kg（组4）处理7周。用睾酮加E2治疗3周后，口服TZP-4238和CMA 4周。在第2组中，前列腺腺体增生，清楚地观察到，溴脱氧尿苷（BrdU）阳性细胞的数量显着增加。与此相反，TZP-4238（第3组）或CMA（第4组）的联合治疗导致腺上皮显著萎缩，BrdU阳性细胞的数量与第2组相比显著减少。此外，谷胱甘肽过氧化物酶（GSH-PO），有效地减少脂质过氧化物在腺上皮细胞的定位显着减少。此外，TZP-4238或CMA联合治疗后，雄激素受体的核免疫染色显着减少。我们的数据表明，TZP-4238是一种有效的甾体雄激素受体拮抗剂，用于预防类固醇诱导的前列腺增生模型中的大鼠前列腺生长。
The effect of a synthetic steroidal anti-androgen, TZP-4238, on steroid-induced rat prostatic hyperplasia was investigated. Male Wistar rats were divided into four experimental groups. Group 1 consisted of intact controls. The other animals were castrated. The castrated animals were treated for 7 weeks with 1) testosterone 1 mg/head plus 17 beta-estradiol (E2) 0.01 mg/head (Group 2), 2) testosterone plus E2 + TZP-4238 8 mg/kg (Group 3) and 3) testosterone plus E2 + chlormadinone acetate (CMA) 20 mg/kg (Group 4). TZP-4238 and CMA were administered orally for 4 weeks after 3 weeks treatment with testosterone plus E2. In group 2, glandular hyperplasia of the prostate was clearly observed, and the number of bromo-deoxyuridine (BrdU)-positive cells showed a significant increase. In contrast, combined treatment with TZP-4238 (Group 3) or CMA (Group 4) produced marked atrophy of the glandular epithelium, and the number of BrdU-positive cells were remarkably decreased compared with Group 2. In addition, the localization of glutathione-peroxidase (GSH-PO) which effectively reduces the lipid peroxides in the glandular epithelial cells was markedly decreased. Furthermore, nuclear immunostaining of androgen receptor was remarkably decreased after combined treatment with TZP-4238 or CMA. Our data indicate that TZP-4238 is a potent steroidal androgen receptor antagonist for the prevention of rat prostatic growth in the steroid-induced prostatic hyperplasia model.
影响因子：
4.8
作者：
SAR, M;LUBAHN, DB;WILSON, EM
通讯作者：
WILSON, EM
影响因子：
--
作者：
Jiann-an Tan;D. R. Joseph;V. Quarmby;D. Lubahn;M. Sar;F. S. French;E. M. Wilson
通讯作者：
Jiann-an Tan;D. R. Joseph;V. Quarmby;D. Lubahn;M. Sar;F. S. French;E. M. Wilson