Bacterial bile metabolising gene abundance in Crohn's, ulcerative colitis and type 2 diabetes metagenomes.
Bacterial bile metabolising gene abundance in Crohn's, ulcerative colitis and type 2 diabetes metagenomes.
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DOI：
10.1371/journal.pone.0115175
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Jones ML
中科院分区：
文献类型：
--
作者：
Labbé A;Ganopolsky JG;Martoni CJ;Prakash S;Jones ML
我们进行了一项分析，以确定胆汁酸修饰基因在炎症性肠病和2型糖尿病患者肠道微生物组中的重要性。我们使用来自人类微生物组计划和MetaHIT联盟的公开可用的宏基因组数据集，并确定了克罗恩病（CD）、溃疡性结肠炎（UC）和2型糖尿病（T2 DM）患病人群粪便细菌中胆盐水解酶基因（bsh）、7 α-脱羟酶基因（adh）和7 α-羟基类固醇脱氢酶基因（hsdh）的丰度。门水平丰度分析显示，相对于健康对照，UC和T2 DM患者中Firmicute衍生的bsh显著降低，但CD患者中未降低。与健康对照相比，在UC和T2 DM患者中也观察到adh和hsdh基因减少，而在CD人群中观察到增加。对bsh基因的进一步分析表明，某些厚壁菌门家族与疾病或健康人群的相关性存在显着差异。从这一观察，我们继续分析BSH蛋白序列，并确定了BSH蛋白簇代表最丰富的菌株在我们的分析厚壁菌bsh基因。相对于健康对照组，在所有疾病状态下，对应于这些蛋白质簇之一的bsh基因的丰度显著降低。该簇包括来源于裂殖酵母科、梭菌科、丹毒丝菌科和瘤胃球菌科的bsh基因。这种宏基因组分析提供了胆汁酸修饰酶在健康和疾病中的重要性的证据。它进一步强调了识别基因和蛋白质簇的重要性，因为相同的基因可能与健康或疾病相关，这取决于表达酶的菌株以及酶本身的差异。
We performed an analysis to determine the importance of bile acid modification genes in the gut microbiome of inflammatory bowel disease and type 2 diabetic patients. We used publicly available metagenomic datasets from the Human Microbiome Project and the MetaHIT consortium, and determined the abundance of bile salt hydrolase gene (bsh), 7 alpha-dehydroxylase gene (adh) and 7-alpha hydroxysteroid dehydrogenase gene (hsdh) in fecal bacteria in diseased populations of Crohn's disease (CD), Ulcerative Colitis (UC) and Type 2 diabetes mellitus (T2DM). Phylum level abundance analysis showed a significant reduction in Firmicute-derived bsh in UC and T2DM patients but not in CD patients, relative to healthy controls. Reduction of adh and hsdh genes was also seen in UC and T2DM patients, while an increase was observed in the CD population as compared to healthy controls. A further analysis of the bsh genes showed significant differences in the correlations of certain Firmicutes families with disease or healthy populations. From this observation we proceeded to analyse BSH protein sequences and identified BSH proteins clusters representing the most abundant strains in our analysis of Firmicute bsh genes. The abundance of the bsh genes corresponding to one of these protein clusters was significantly reduced in all disease states relative to healthy controls. This cluster includes bsh genes derived from Lachospiraceae, Clostridiaceae, Erysipelotrichaceae and Ruminococcaceae families. This metagenomic analysis provides evidence of the importance of bile acid modifying enzymes in health and disease. It further highlights the importance of identifying gene and protein clusters, as the same gene may be associated with health or disease, depending on the strains expressing the enzyme, and differences in the enzymes themselves.
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影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1038/oby.2009.102
发表时间：
2009-09
期刊：
Obesity (Silver Spring, Md.)
影响因子：
--
作者：
Patti ME;Houten SM;Bianco AC;Bernier R;Larsen PR;Holst JJ;Badman MK;Maratos-Flier E;Mun EC;Pihlajamaki J;Auwerx J;Goldfine AB
通讯作者：
Goldfine AB
影响因子：
3.7
作者：
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通讯作者：
Jansson JK
DOI：
10.1152/ajpendo.00137.2010
发表时间：
2010-07-01
影响因子：
5.1
作者：
Knop, Filip K.
通讯作者：
Knop, Filip K.
CLOVR：使用云计算从桌面上自动化和便携式序列分析的虚拟机。
影响因子：
3
作者：
Angiuoli SV;Matalka M;Gussman A;Galens K;Vangala M;Riley DR;Arze C;White JR;White O;Fricke WF
通讯作者：
Fricke WF