Integrated metagenomics/metaproteomics reveals human host-microbiota signatures of Crohn's disease.
Integrated metagenomics/metaproteomics reveals human host-microbiota signatures of Crohn's disease.
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DOI：
10.1371/journal.pone.0049138
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Jansson JK
中科院分区：
文献类型：
--
作者：
Erickson AR;Cantarel BL;Lamendella R;Darzi Y;Mongodin EF;Pan C;Shah M;Halfvarson J;Tysk C;Henrissat B;Raes J;Verberkmoes NC;Fraser CM;Hettich RL;Jansson JK
克罗恩病（CD）是一种病因复杂的炎症性肠病，尽管肠道微生物群的生态失调与CD相关的慢性免疫介导的炎症有关。在这里，我们结合了鸟枪宏基因组学和元蛋白组学方法，来识别来自六对健康双胞胎或在回肠（ICD）或结肠（CCD）中患有CD的双胞胎粪便样本中CD的潜在功能特征。这些组学方法的整合揭示了主要区分ICD与健康受试者的几个基因、蛋白质和途径，包括ICD中许多蛋白质的耗尽。此外，ICD表型与细菌碳水化合物代谢、细菌-宿主相互作用以及人类宿主分泌酶的改变相关。这种生态系统生物学方法强调了肠道微生物群与克罗恩病病理生理学中的功能改变之间的联系，并有助于鉴定新的诊断靶点和疾病特异性生物标志物。
Crohn's disease (CD) is an inflammatory bowel disease of complex etiology, although dysbiosis of the gut microbiota has been implicated in chronic immune-mediated inflammation associated with CD. Here we combined shotgun metagenomic and metaproteomic approaches to identify potential functional signatures of CD in stool samples from six twin pairs that were either healthy, or that had CD in the ileum (ICD) or colon (CCD). Integration of these omics approaches revealed several genes, proteins, and pathways that primarily differentiated ICD from healthy subjects, including depletion of many proteins in ICD. In addition, the ICD phenotype was associated with alterations in bacterial carbohydrate metabolism, bacterial-host interactions, as well as human host-secreted enzymes. This eco-systems biology approach underscores the link between the gut microbiota and functional alterations in the pathophysiology of Crohn's disease and aids in identification of novel diagnostic targets and disease specific biomarkers.
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