Trypanosoma amblyommi sp. nov. (Protozoa: Kinetoplastida) isolated from Amblyomma brasiliense (Acari: Ixodidae) ticks in Rio de Janeiro, Brazil.
Trypanosoma amblyommi sp. nov. (Protozoa: Kinetoplastida) isolated from Amblyomma brasiliense (Acari: Ixodidae) ticks in Rio de Janeiro, Brazil.
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温和锥虫 sp.
DOI：
10.1017/pao.2017.17
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Marotta CR
中科院分区：
文献类型：
--
作者：
Marotta CR
锥虫属的寄生虫是锥虫科的单细胞鞭毛微生物。本研究描述了一株自然感染微小扇头蜱的锥虫属分离株，通过分子、形态和生物学分析对其进行了鉴定。从R.在蜱细胞系IDE8的培养物中，分离后的菌株P1RJ在L15B培养基中30、32和34 °C下生长良好。新的锥虫在30 °C下在L15B中的无菌培养物中保持稳定超过14代，并成功地冷冻保存和复苏。对随机选择的发育形式进行形态测定分析。经18S rRNA和24 S α rDNA序列分析，证实P1RJ为锥虫属的一个新种。将所描述的核苷酸序列提交至Genbank。P1RJ菌株的致病性、在脊椎动物宿主中的参与、流行病学、发育周期和传播机制仍不清楚。因此，更多的研究将是必要的，以确定生命周期方面的锥虫，我们建议的名称锥虫rhipicephalis sp。
Parasites of the genus Trypanosoma are unicellular flagellated microorganisms of the Trypanosomatidae. This study describes an isolate of the genus Trypanosoma naturally infecting Rhipicephalus microplus ticks, characterized through molecular, morphological and biological analysis. Trypanosome cultures, designated strain P1RJ, were obtained by isolation from R. microplus haemolymph in cultures of the tick cell line IDE8. After isolation, strain P1RJ grew well axenically in L15B medium at temperatures of 30, 32 and 34 °C. The new trypanosome remained stable in axenic culture over 14 passages in L15B at 30 °C and was successfully cryopreserved and resuscitated. Morphometric analysis was performed on randomly selected developmental forms. 18S rRNA and 24Sα rDNA sequence analyses confirmed that strain P1RJ is a new species of the genus Trypanosoma. The nucleotide sequences described were submitted to Genbank. Pathogenicity, involvement in vertebrate hosts, epidemiology, developmental cycle and transmission mechanisms of strain P1RJ are still unknown. Therefore, more studies will be necessary to determine life cycle aspects of this trypanosome, for which we propose the name Trypanosoma rhipicephalis sp. nov.
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潘塔纳尔湿地伊氏锥虫的测量。
影响因子：
2.8
作者：
L. Ramirez;A. Dávila;A. Victorio;R. Silva;V. Trajano;A. Jansen
通讯作者：
A. Jansen
影响因子：
2.4
作者：
Madeira, M. F.;Sousa, M. A.;Marzochi, M. C. A.
通讯作者：
Marzochi, M. C. A.
DOI：
--
发表时间：
1936
期刊：
影响因子：
--
作者：
H. D. B. Aragão
通讯作者：
H. D. B. Aragão
影响因子：
2.2
作者：
MUNDERLOH, UG;KURTTI, TJ
通讯作者：
KURTTI, TJ
泰勒锥虫：采采蝇和脊椎动物细胞培养系统的体外培养。
影响因子：
4
作者：
M. Wink
通讯作者：
M. Wink