Transverse susceptibility as a biosensor for detection of Au-Fe₃O₄ nanoparticle-embedded human embryonic kidney cells.
Transverse susceptibility as a biosensor for detection of Au-Fe₃O₄ nanoparticle-embedded human embryonic kidney cells.
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DOI：
10.3390/s130708490
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发表时间：
2013-07-03
期刊：
影响因子：
--
通讯作者：
Srikanth H
中科院分区：
文献类型：
--
作者：
Huls NF;Phan MH;Kumar A;Mohapatra S;Mohapatra S;Mukherjee P;Srikanth H
我们证明了使用基于灵敏的自共振隧道二极管振荡器的射频横向磁化率(TS)技术作为生物传感器来检测已经摄取磁性纳米颗粒的癌细胞的可能性。这项技术可以在10 MHz的数量级上检测到频率的变化。因此，当放置在TS探测器的样本空间内时，已经吸收纳米粒子的小样本细胞可以产生磁性纳米粒子的信号特征。为了验证这一概念，用胶束法制备了包覆Au(平均尺寸为60 nm)的Fe3O4纳米粒子，并将这些纳米粒子以不同浓度的缓冲液以0.05、0.1、0.5和1 mg/mL的浓度引入到介质中，通过吞噬作用被人胚胎肾细胞摄取。虽然Au-Fe3O4纳米粒子的最高浓度(1 mg/mL)给出了最强的TS信号，但值得注意的是，在低至0.1 mg/mL的浓度下，仍然可以检测到纳米粒子的TS信号。
We demonstrate the possibility of using a radio-frequency transverse susceptibility (TS) technique based on a sensitive self-resonant tunnel-diode oscillator as a biosensor for detection of cancer cells that have taken up magnetic nanoparticles. This technique can detect changes in frequency on the order of 10 Hz in 10 MHz. Therefore, a small sample of cells that have taken up nanoparticles when placed inside the sample space of the TS probe can yield a signal characteristic of the magnetic nanoparticles. As a proof of the concept, Fe3O4 nanoparticles coated with Au (mean size ∼60 nm) were synthesized using a micellar method and these nanoparticles were introduced to the medium at different concentrations of 0.05, 0.1, 0.5, and 1 mg/mL buffer, where they were taken up by human embryonic kidney (HEK) cells via phagocytosis. While the highest concentration of Au-Fe3O4 nanoparticles (1 mg/mL) was found to give the strongest TS signal, it is notable that the TS signal of the nanoparticles could still be detected at concentrations as low as 0.1 mg/mL.
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影响因子：
12.6
作者：
Kurlyandskaya, G;Levit, V
通讯作者：
Levit, V
影响因子：
2.7
作者：
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通讯作者：
Lees, Martin
影响因子：
3.7
作者：
Frey, N. A.;Srinath, S.;Gupta, A.
通讯作者：
Gupta, A.
影响因子：
3.2
作者：
Morales, M. B.;Phan, M. H.;Srikanth, H.
通讯作者：
Srikanth, H.
DOI：
10.3390/s91007785
发表时间：
2009
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Herrera-May AL;Aguilera-Cortés LA;García-Ramírez PJ;Manjarrez E
通讯作者：
Manjarrez E