Immunologic environment influences macrophage response to Porphyromonas gingivalis.
Immunologic environment influences macrophage response to Porphyromonas gingivalis.
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DOI：
10.1111/omi.12168
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发表时间：
2017-06
影响因子：
3.7
通讯作者：
Gibson FC 3rd
中科院分区：
文献类型：
--
作者：
Papadopoulos G;Shaik-Dasthagirisaheb YB;Huang N;Viglianti GA;Henderson AJ;Kantarci A;Gibson FC 3rd
巨噬细胞在表型和功能上适应宿主组织微环境中的细胞因子平衡。最近的研究表明，巨噬细胞在感染引起的口腔骨丢失的发展中起着重要但知之甚少的作用。我们假设，巨噬细胞适应炎症信号之前遇到的病原体相互作用将显着影响随后的免疫反应，这些细胞的关键口腔致病菌牙龈卟啉单胞菌。采用经典活化（M1）和交替活化（M2）的小鼠骨髓源性巨噬细胞（BMDM），我们观察到巨噬细胞在牙龈卟啉单胞菌攻击前的免疫活化决定了炎症相关分子表达的表型特异性变化，这些炎症相关分子对感知和调节宿主对细菌感染的反应很重要，包括Toll样受体（TLR）2和4，CD 14，CD 18和CD 11b（一起构成CR 3）、MHCII、CD 80和CD 86。M2细胞对牙龈卟啉单胞菌的反应比M1细胞更强，TNF-α、IL-6、MCP-1、MIP-1α、RANTES和KC的表达也更高。M1 BMDM菌株对牙龈卟啉单胞菌的IL-10表达水平高于M2 BMDM菌株。在功能上，我们观察到M2 BMDM β比M1 BMDM β更稳健地结合牙龈卟啉单胞菌。这些数据描述了巨噬细胞偏斜在对牙龈卟啉单胞菌的细胞免疫应答的后续发展中的重要贡献。
Macrophages adapt both phenotypically and functionally to the cytokine balance in host tissue microenvironments. Recent studies established that macrophages contribute an important yet poorly understood role in the development of infection-elicited oral bone loss. We hypothesized that macrophage adaptation to inflammatory signals encountered prior to pathogen interaction would significantly influence the subsequent immune response of these cells to the keystone oral pathobiont Porphyromonas gingivalis. Employing classically activated (M1) and alternatively activated (M2) murine bone marrow-derived macrophage (BMDM∅), we observed that immunologic activation of macrophages prior to P. gingivalis challenge dictated phenotype-specific changes in the expression of inflammation-associated molecules important to sensing and tuning host response to bacterial infection including Toll-like receptors (TLR) 2 and 4, CD14, CD18 and CD11b (together comprising CR3), MHCII, CD80, and CD86. M2 cells responded to P. gingivalis with higher expression of TNF-α, IL-6, MCP-1, MIP-1α, RANTES, and KC than M1 cells. M1 BMDM∅ expressed higher levels of IL-10 to P. gingivalis than M2 BMDM∅. Functionally, we observed that M2 BMDM∅ bound P. gingivalis more robustly than M1 BMDM∅. These data describe an important contribution of macrophage skewing in the subsequent development of the cellular immune response to P. gingivalis.
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影响因子：
3.7
作者：
Foey AD;Crean S
通讯作者：
Crean S
影响因子：
4.4
作者：
Burns, Elia;Eliyahu, Tal;Nussbaum, Gabriel
通讯作者：
Nussbaum, Gabriel
影响因子：
4.3
作者：
CHARON, J;TOTO, PD;GARGIULO, AW
通讯作者：
GARGIULO, AW
影响因子：
3.4
作者：
Hajishengallis, George;Tapping, Richard I.;Yoshimura, Fuminobu
通讯作者：
Yoshimura, Fuminobu
DOI：
10.1073/pnas.1402740111
发表时间：
2014-04-08
影响因子：
11.1
作者：
Boisvert, Heike;Lorand, Laszlo;Duncan, Margaret J.
通讯作者：
Duncan, Margaret J.