Increased insulin-like growth factor binding protein-2 (IGFBP-2) gene expression and protein production lead to high IGFBP-2 content in malignant ovarian cyst fluid.
Increased insulin-like growth factor binding protein-2 (IGFBP-2) gene expression and protein production lead to high IGFBP-2 content in malignant ovarian cyst fluid.
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DOI：
10.1038/bjc.1996.206
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发表时间：
1996-05
影响因子：
8.8
通讯作者：
Karasik, A
中科院分区：
文献类型：
--
作者：
Kanety, H;Kattan, M;Goldberg, I;Kopolovic, J;Ravia, J;Menczer, J;Karasik, A
胰岛素样生长因子-I（IGF-I），其受体和IGF-binding proteins（IGFBPs）的卵巢癌细胞的表达和体外对这些细胞的促有丝分裂作用，表明IGF-I可能在人卵巢癌的调节中发挥作用。我们最近发现IGFBP-2在体内恶性卵巢囊肿液中明显升高。为了确定这些囊肿液中IGFBP-2增加的起源，研究了14例恶性和4例良性上皮性卵巢肿瘤中IGFBP-2的基因表达和蛋白含量。IGFBP-2 mRNA在所有卵巢标本中均检测到，恶性肿瘤中IGFBP-2 mRNA的表达是良性肿瘤的2- 30倍。在恶性组织中，IGFBP-2 mRNA水平与肿瘤的侵袭性相关，并且在侵袭性肿瘤中高于那些具有边界病理学的肿瘤。Southern印迹分析显示，无论其性质如何，卵巢肿瘤的DNA样本中IGFBP-2基因均未扩增。IGFBP-2是组织提取物中的主要结合蛋白，通过Western配体印迹和免疫印迹测定，并且在恶性肿瘤中显著高于良性肿瘤。肿瘤切片中IGFBP-2的免疫组化检测进一步支持了这些发现。我们的数据表明，增加局部生产的肿瘤在体内是负责增加IGFBP-2水平的囊肿液洗澡卵巢恶性肿瘤。这可能是IGF-I对卵巢癌增殖作用的一种自分泌调节机制。
Expression of insulin-like growth factor-I (IGF-I), its receptor and IGF-binding proteins (IGFBPs) by ovarian cancer cells and its mitogenic effect on these cells in vitro, suggest that IGF-I may have a role in regulation of human ovarian cancer. We have recently shown IGFBP-2 to be markedly elevated in malignant ovarian cyst fluid in vivo. To identify the origin of increased IGFBP-2 in these cyst fluids, the gene expression and protein content of IGFBP-2 were investigated in 14 malignant and four benign epithelial ovarian neoplasms. IGFBP-2 mRNA was detected in all ovarian specimens and was 2- to 30-fold higher in malignant than in benign neoplasms. Within the malignant tissues IGFBP-2 mRNA levels correlated with the aggressiveness of the tumour and were higher in invasive tumours than in those with borderline pathology. Southern blot analysis revealed no amplification of IGFBP-2 gene in the DNA samples from ovarian tumours regardless of their nature. IGFBP-2 was the major binding protein in tissue extracts, as measured by both Western ligand blotting and immunoblotting, and was significantly higher in malignant than in benign neoplasms. These findings were further supported by immunohistochemical detection of IGFBP-2 in tumour sections. Our data suggest that increased local production by the tumour in vivo is responsible for the increased IGFBP-2 levels in the cyst fluid bathing the ovarian malignancy. This may represent an autocrine regulatory mechanism for IGF-I proliferative effect of ovarian cancer.
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DOI：
10.1073/pnas.88.17.7481
发表时间：
1991-09-01
影响因子：
11.1
作者：
JONES, JI;DERCOLE, AJ;CLEMMONS, DR
通讯作者：
CLEMMONS, DR
影响因子：
39.2
作者：
LEROITH, D;BASERGA, R;ROBERTS, CT
通讯作者：
ROBERTS, CT
影响因子：
8.8
作者：
REEVE, JG;PAYNE, JA;BLEEHEN, NM
通讯作者：
BLEEHEN, NM
影响因子：
5.6
作者：
CHEN, JC;SHAO, ZM;FONTANA, JA
通讯作者：
FONTANA, JA
影响因子：
15.9
作者：
CONOVER, CA;KIEFER, MC;ZAPF, J
通讯作者：
ZAPF, J