Isolation of Oct4-expressing extraembryonic endoderm precursor cell lines.
Isolation of Oct4-expressing extraembryonic endoderm precursor cell lines.
复制标题
表达OCT4的内胚层前体细胞系的分离。
DOI：
10.1371/journal.pone.0007216
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发表时间：
2009-09-28
期刊：
影响因子：
3.7
通讯作者：
Binas B
中科院分区：
文献类型：
--
作者：
Debeb BG;Galat V;Epple-Farmer J;Iannaccone S;Woodward WA;Bader M;Iannaccone P;Binas B
胚胎外内胚层（ExEn）定义了卵黄囊，这是一组为哺乳动物胚胎提供基本支持的膜。最近的研究结果表明，承诺的ExEn前体已经存在于胚胎内细胞团（ICM）中，作为一组细胞与密切相关的外胚层前体混合在一起。所有ICM细胞都含有Oct4，这是一个关键的转录因子，在桑葚胚期首次表达。体外实验中，外胚层前体细胞最接近于维持Oct4表达的胚胎干（ES）细胞系，但尚不清楚类似的ExEn前体细胞系是否表达Oct4。在这里，我们报道了永久增殖表达oct4的大鼠细胞系（“XEN-P细胞系”）的分离和特性，这些细胞系与ExEn前体非常相似。我们从囊胚中分离出XEN-P细胞系，并通过电泳和基因表达测定以及胚胎注射对其进行鉴定。与ES细胞一样，XEN-P细胞高水平表达Oct4和SSEA1，其生长受到白血病抑制因子的刺激，但它们表达的不是外胚层决定因子Nanog，而是外胚层决定因子Gata6和Gata4。此外，它们缺乏更分化的原始/内脏和壁层ExEn阶段的特征标记，但在体外只能分化为这些阶段，并在体内促进这些阶段。我们的研究结果(i)强烈表明ExEn前体是一个自我再生的实体，（ii）表明活跃的Oct4基因表达（转录加翻译）是其分子特性的一部分，（iii）提供了早期ExEn分化的体外模型。
The extraembryonic endoderm (ExEn) defines the yolk sac, a set of membranes that provide essential support for mammalian embryos. Recent findings suggest that the committed ExEn precursor is present already in the embryonic Inner Cell Mass (ICM) as a group of cells that intermingles with the closely related epiblast precursor. All ICM cells contain Oct4, a key transcription factor that is first expressed at the morula stage. In vitro, the epiblast precursor is most closely represented by the well-characterized embryonic stem (ES) cell lines that maintain the expression of Oct4, but analogous ExEn precursor cell lines are not known and it is unclear if they would express Oct4. Here we report the isolation and characterization of permanently proliferating Oct4-expressing rat cell lines (“XEN-P cell lines”), which closely resemble the ExEn precursor. We isolated the XEN-P cell lines from blastocysts and characterized them by plating and gene expression assays as well as by injection into embryos. Like ES cells, the XEN-P cells express Oct4 and SSEA1 at high levels and their growth is stimulated by leukemia inhibitory factor, but instead of the epiblast determinant Nanog, they express the ExEn determinants Gata6 and Gata4. Further, they lack markers characteristic of the more differentiated primitive/visceral and parietal ExEn stages, but exclusively differentiate into these stages in vitro and contribute to them in vivo. Our findings (i) suggest strongly that the ExEn precursor is a self-renewable entity, (ii) indicate that active Oct4 gene expression (transcription plus translation) is part of its molecular identity, and (iii) provide an in vitro model of early ExEn differentiation.
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DOI：
10.1002/tera.1420410403
发表时间：
1990-04-01
期刊：
TERATOLOGY
影响因子：
--
作者：
JOLLIE, WP
通讯作者：
JOLLIE, WP
影响因子：
64.8
作者：
Brons, I. Gabrielle M.;Smithers, Lucy E.;Vallier, Ludovic
通讯作者：
Vallier, Ludovic
影响因子：
82.9
作者：
Fändrich, F;Lin, XB;Binas, B
通讯作者：
Binas, B
影响因子：
64.5
作者：
Li P;Tong C;Mehrian-Shai R;Jia L;Wu N;Yan Y;Maxson RE;Schulze EN;Song H;Hsieh CL;Pera MF;Ying QL
通讯作者：
Ying QL
影响因子：
56.9
作者：
Lois, C;Hong, EJ;Baltimore, D
通讯作者：
Baltimore, D