Hydrogels derived from demineralized and decellularized bone extracellular matrix.
Hydrogels derived from demineralized and decellularized bone extracellular matrix.
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DOI：
10.1016/j.actbio.2013.04.029
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发表时间：
2013-08
影响因子：
9.7
通讯作者：
White, L. J.
中科院分区：
文献类型：
--
作者：
Sawkins, M. J.;Bowen, W.;Dhadda, P.;Markides, H.;Sidney, L. E.;Taylor, A. J.;Rose, F. R. A. J.;Badylak, S. F.;Shakeshehh, K. M.;White, L. J.
哺乳动物组织的细胞外基质（ECM）已被分离、脱细胞化并用作支架以促进许多组织的修复和重建。最近的研究表明，与异源组织相比，当ECM支架来源于位点特异性同源组织时，发生上级功能和复杂组织形成。本研究的目的是将严格的脱细胞过程应用于从牛骨制备的脱钙骨基质（DBM），并表征所得ECM材料和DBM本身的结构和组成。此外，我们试图产生可被诱导形成水凝胶的可溶形式的DBM和ECM。目前用于治疗骨缺损的DBM颗粒的临床递送需要将颗粒掺入载液中。已报道了不同载液在成骨活性、炎症和肾毒性方面的差异。使用水凝胶形式的DBM或ECM可以减少对载体液体的需要。DBM和ECM水凝胶表现出与成核和生长机制一致的S形凝胶化动力学，ECM水凝胶的特征在于储能模量比DBM水凝胶低。与I型胶原和DBM水凝胶相比，ECM水凝胶实现了小鼠原代颅骨细胞的增殖增强。这些结果表明，DBM和ECM水凝胶具有不同的结构、机械和生物学特性，并且具有临床递送的潜力，而不需要载体液体。
The extracellular matrix (ECM) of mammalian tissues has been isolated, decellularized and utilized as a scaffold to facilitate the repair and reconstruction of numerous tissues. Recent studies have suggested that superior function and complex tissue formation occurred when ECM scaffolds were derived from site-specific homologous tissues compared with heterologous tissues. The objectives of the present study were to apply a stringent decellularization process to demineralized bone matrix (DBM), prepared from bovine bone, and to characterize the structure and composition of the resulting ECM materials and DBM itself. Additionally, we sought to produce a soluble form of DBM and ECM which could be induced to form a hydrogel. Current clinical delivery of DBM particles for treatment of bone defects requires incorporation of the particles within a carrier liquid. Differences in osteogenic activity, inflammation and nephrotoxicity have been reported with various carrier liquids. The use of hydrogel forms of DBM or ECM may reduce the need for carrier liquids. DBM and ECM hydrogels exhibited sigmoidal gelation kinetics consistent with a nucleation and growth mechanism, with ECM hydrogels characterized by lower storage moduli than the DBM hydrogels. Enhanced proliferation of mouse primary calvarial cells was achieved on ECM hydrogels, compared with collagen type I and DBM hydrogels. These results show that DBM and ECM hydrogels have distinct structural, mechanical and biological properties and have the potential for clinical delivery without the need for carrier liquids.
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影响因子：
3.1
作者：
DeQuach JA;Lin JE;Cam C;Hu D;Salvatore MA;Sheikh F;Christman KL
通讯作者：
Christman KL
影响因子：
4.5
作者：
Markel DC;Guthrie ST;Wu B;Song Z;Wooley PH
通讯作者：
Wooley PH
DOI：
10.1126/science.1189345
发表时间：
2010-07-30
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Petersen TH;Calle EA;Zhao L;Lee EJ;Gui L;Raredon MB;Gavrilov K;Yi T;Zhuang ZW;Breuer C;Herzog E;Niklason LE
通讯作者：
Niklason LE
影响因子：
14
作者：
Freytes, Donald O.;Martin, Jeffrey;Badylak, Stephen F.
通讯作者：
Badylak, Stephen F.
影响因子：
3.3
作者：
Hadjipanayi, E.;Mudera, V.;Brown, R. A.
通讯作者：
Brown, R. A.