Cerenkov radiation energy transfer (CRET) imaging: a novel method for optical imaging of PET isotopes in biological systems.
Cerenkov radiation energy transfer (CRET) imaging: a novel method for optical imaging of PET isotopes in biological systems.
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DOI：
10.1371/journal.pone.0013300
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发表时间：
2010-10-11
期刊：
影响因子：
3.7
通讯作者：
Piwnica-Worms D
中科院分区：
文献类型：
--
作者：
Dothager RS;Goiffon RJ;Jackson E;Harpstrite S;Piwnica-Worms D
正电子发射断层扫描 (PET) 可以对小动物和人类中用正电子 (β+) 发射放射性核素标记的放射性药物示踪剂的分布进行灵敏、非侵入性的分析。在 β+ 衰变时，高能 β+ 粒子的初始速度可以瞬间超过组织中的光速，产生可通过光学成像检测到的切伦科夫辐射，但在生物体中被高度吸收。为了改善生物系统中切伦科夫辐射的光学成像，我们证明来自 PET 同位素 64Cu 和 18F 衰变的切伦科夫辐射可以通过能量转移到高斯托克斯位移量子纳米粒子 (Qtracker705) 进行光谱耦合，以产生高度红移的光子发射。 99mTc（一种主要发射 γ 的同位素）未检测到有效的能量转移。与生物发光共振能量转移（BRET）和荧光共振能量转移（FRET）类似，本文将切伦科夫辐射能量转移（CRET）比率定义为以荧光团发射为中心的光谱窗口内检测到的光除以切伦科夫辐射发射的光谱窗口内检测到的光的归一化商，以量化成像信号。含有Qtracker705纳米颗粒和[18F]FDG的溶液的光学图像显示体外CRET比率高达8.8±1.1，而静脉注射[18F]FDG后皮下假瘤小鼠用Qtracker705浸渍的图像显示体内CRET比率高达3.5±0.3。定量 CRET 成像可为 PET 放射性药物和生物材料、组织和活体动物中的其他同位素提供各种新颖的光学成像应用和激活策略。
Positron emission tomography (PET) allows sensitive, non-invasive analysis of the distribution of radiopharmaceutical tracers labeled with positron (β+)-emitting radionuclides in small animals and humans. Upon β+ decay, the initial velocity of high-energy β+ particles can momentarily exceed the speed of light in tissue, producing Cerenkov radiation that is detectable by optical imaging, but is highly absorbed in living organisms. To improve optical imaging of Cerenkov radiation in biological systems, we demonstrate that Cerenkov radiation from decay of the PET isotopes 64Cu and 18F can be spectrally coupled by energy transfer to high Stokes-shift quantum nanoparticles (Qtracker705) to produce highly red-shifted photonic emissions. Efficient energy transfer was not detected with 99mTc, a predominantly γ-emitting isotope. Similar to bioluminescence resonance energy transfer (BRET) and fluorescence resonance energy transfer (FRET), herein we define the Cerenkov radiation energy transfer (CRET) ratio as the normalized quotient of light detected within a spectral window centered on the fluorophore emission divided by light detected within a spectral window of the Cerenkov radiation emission to quantify imaging signals. Optical images of solutions containing Qtracker705 nanoparticles and [18F]FDG showed CRET ratios in vitro as high as 8.8±1.1, while images of mice with subcutaneous pseudotumors impregnated with Qtracker705 following intravenous injection of [18F]FDG showed CRET ratios in vivo as high as 3.5±0.3. Quantitative CRET imaging may afford a variety of novel optical imaging applications and activation strategies for PET radiopharmaceuticals and other isotopes in biomaterials, tissues and live animals.
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影响因子：
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通讯作者：
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