EDB Gene Cluster-Dependent Indole Production Is Responsible for the Ability of Pseudomonas fluorescens NZI7 to Repel Grazing by Caenorhabditis elegans.
EDB Gene Cluster-Dependent Indole Production Is Responsible for the Ability of Pseudomonas fluorescens NZI7 to Repel Grazing by Caenorhabditis elegans.
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DOI：
10.1021/acs.jnatprod.1c01046
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发表时间：
2022-03-25
影响因子：
5.1
通讯作者：
Mahmud, Taifo
中科院分区：
文献类型：
--
作者：
Zhou, Wei;Vergis, John;Mahmud, Taifo
“EDB”（来自“可食用”）基因簇是在许多细菌和藻类中发现的ebo基因簇的变体，允许荧光假单胞菌NZI 7（此处称为“NZI 7”）排斥线虫秀丽隐杆线虫的摄食。这种表型的机制尚不清楚。在这里，我们报告说，EDB簇参与色氨酸转化为（1H-吲哚-3-基）-氧代乙酰胺，吲哚-3-醛，和其他吲哚衍生化合物。NZI 7中EDB基因的失活导致突变体缺乏分泌吲哚衍生化合物的能力以及排斥C.优雅NZI 7 EDB簇在E.在含有2 mM L-色氨酸的基本M9培养基中培养的大肠杆菌也释放吲哚衍生物，包括胆甾醇、3-（羟基乙酰基）吲哚、colletotryptin E和两种新的二聚吲哚。NZI 7 EDB簇在E.大肠杆菌，培养在最低限度的M9培养基和缺乏色氨酸，不产生可检测水平的吲哚衍生物。（1H-吲哚-3-基）-氧代乙酰胺和吲哚-3-醛均表现出驱避活性。揭示了荧光假单胞菌NZI 7对线虫的驱避机制。优雅
The “EDB” (from “edible”) gene cluster, a variant of the ebo cluster of genes found in many bacteria and algae, allows Pseudomonas fluorescens NZI7 (referred to here as “NZI7”) to repel grazing by the nematode Caenorhabditis elegans. The mechanism underlying this phenotype is unknown. Here we report that the EDB cluster is involved in the conversion of tryptophan to (1H-indol-3-yl)-oxoacetamide, indole 3-aldehyde, and other indole-derived compounds. Inactivation of the EDB genes in NZI7 resulted in mutants that lack the ability to excrete indole-derived compounds as well as the ability to repel C. elegans. Heterologous expression of the NZI7 EDB cluster in E. coli cultivated in minimal M9 medium containing 2 mM L-tryptophan also released indole derivatives including tryptophol, 3-(hydroxyacetyl)indole, colletotryptin E and two new dimeric indoles. Expression of the NZI7 EDB cluster in E. coli, cultured in minimal M9 medium and lacking tryptophan, did not produce detectable levels of indole derivatives. Both (1H-indol-3-yl)-oxoacetamide and indole 3-aldehyde showed repellent activity against C. elegans, revealing the mechanism underlying the ability of P. fluorescens NZI7 to repel grazing by C. elegans.
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影响因子：
2.9
作者：
Albuquerque P;Casadevall A
通讯作者：
Casadevall A
化学防御机制和印度太平洋霍洛库的生态意义。
DOI：
10.3390/molecules25204808
发表时间：
2020-10-19
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Kamyab E;Rohde S;Kellermann MY;Schupp PJ
通讯作者：
Schupp PJ
影响因子：
6.4
作者：
Klicki, Kevin;Ferreira, Daniela;Garcia-Pichel, Ferran
通讯作者：
Garcia-Pichel, Ferran
影响因子：
7.4
作者：
Boettcher, Christoph;Chapman, Alexandra;Glawischnig, Erich
通讯作者：
Glawischnig, Erich
Colletotryptins A-F，来自内生真菌 Colletotrichum sp. 的新型二聚色氨酸衍生物。
影响因子：
3.4
作者：
Shao, Lulu;Wu, Ping;Wei, Xiaoyi
通讯作者：
Wei, Xiaoyi