Engineering Aligned Skeletal Muscle Tissue Using Decellularized Plant-Derived Scaffolds.
Engineering Aligned Skeletal Muscle Tissue Using Decellularized Plant-Derived Scaffolds.
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DOI：
10.1021/acsbiomaterials.0c00058
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发表时间：
2020-05-11
影响因子：
5.8
通讯作者：
Feinberg AW
中科院分区：
文献类型：
--
作者：
Cheng YW;Shiwarski DJ;Ball RL;Whitehead KA;Feinberg AW
为了实现组织和功能，工程组织需要支持细胞粘附、排列、生长和分化的支架。对于骨骼肌组织工程，去细胞化已经成为制造保留生物结构的3D支架的方法。虽然许多脱细胞方法集中于利用动物肌肉作为起始材料，但脱细胞植物是高度结构化的富含纤维素的支架的潜在来源。在这里，我们评估了各种脱细胞植物支架促进小鼠和人类肌肉细胞排列和分化的潜力。在对一系列水果和蔬菜进行脱细胞处理后，我们发现大葱支架具有适当的表面形貌，用于产生高度融合和对齐的C2C12和人骨骼肌细胞（HSMCs）。大葱纤维素支架的形貌包含一个重复的凹槽图案，大约20 μm宽× 10 μm深。绿色洋葱的外部白色部分具有引导C2C12细胞分化成对齐的肌管的微观结构。C2C12和HSMC对齐的定量分析显示，与2D各向同性对照相比，几乎完全的各向异性组织。我们的研究结果表明，脱细胞的绿色洋葱纤维素支架，特别是从外部白色灯泡段，提供了一个简单和低成本的基板工程对齐的人骨骼肌。
To achieve organization and function, engineered tissues require a scaffold that supports cell adhesion, alignment, growth, and differentiation. For skeletal muscle tissue engineering, decellularization has been an approach for fabricating 3D scaffolds that retain biological architecture. While many decellularization approaches are focused on utilizing animal muscle as the starting material, decellularized plants are a potential source of highly structured cellulose-rich scaffolds. Here, we assessed the potential for a variety of decellularized plant scaffolds to promote mouse and human muscle cell alignment and differentiation. After decellularizing a range of fruits and vegetables, we identified the green-onion scaffold to have appropriate surface topography for generating highly confluent and aligned C2C12 and human skeletal muscle cells (HSMCs). The topography of the green-onion cellulose scaffold contained a repeating pattern of grooves that are approximately 20 μm wide by 10 μm deep. The outer white section of the green onion had a microstructure that guided C2C12 cell differentiation into aligned myotubes. Quantitative analysis of C2C12 and HSMC alignment revealed an almost complete anisotropic organization compared to 2D isotropic controls. Our results demonstrate that the decellularized green onion cellulose scaffolds, particularly from the outer white bulb segment, provide a simple and low-cost substrate to engineer aligned human skeletal muscle.
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影响因子：
3.7
作者：
Palchesko RN;Zhang L;Sun Y;Feinberg AW
通讯作者：
Feinberg AW
影响因子：
3.8
作者：
Duffy RM;Sun Y;Feinberg AW
通讯作者：
Feinberg AW
影响因子：
14
作者：
Feinberg, Adam W.;Alford, Patrick W.;Jin, Hongwei;Ripplinger, Crystal M.;Werdich, Andreas A.;Sheehy, Sean P.;Grosberg, Anna;Parker, Kevin Kit
通讯作者：
Parker, Kevin Kit
通过表观遗传学重编程和支架操作实现肌肉组织工程和再生。
影响因子：
4.6
作者：
Tan SJ;Fang JY;Wu Y;Yang Z;Liang G;Han B
通讯作者：
Han B
影响因子：
4.9
作者：
Riboldi, S. A.;Sadr, N.;Mantero, S.
通讯作者：
Mantero, S.