Effect of early exposure to ethanol on the protein and DNA contents of specific brain regions in the rat
Effect of early exposure to ethanol on the protein and DNA contents of specific brain regions in the rat
复制标题
早期接触乙醇对大鼠特定脑区蛋白质和DNA含量的影响
DOI：
10.1016/0006-8993(96)00651-8
复制
发表时间：
1996
期刊：
影响因子：
2.9
通讯作者：
Michael W. Miller
中科院分区：
文献类型：
--
作者：
Michael W. Miller
胎儿酒精综合征的一个标志是小脑畸形。小脑畸形可能是由于细胞数量减少或神经元数量减少，或两者兼而有之。由于CNS结构沿着沿着不同的时间线发展，因此每个结构对乙醇的脆弱性窗口可能不同。因此，出生前接触乙醇后的大脑生长与出生后接触乙醇进行了比较。大鼠在产前（妊娠第6天至第21天，通过妊娠母鼠经口摄入）或产后（产后第4天至第12天，通过插管）暴露于乙醇。出生前或出生后暴露于乙醇的大鼠的每日峰值血液乙醇浓度平均值分别为146 ± 16和127 ± 18 mg/dl。在出生前和出生后条件下生成成对喂养的对照大鼠。对21日龄大鼠的6个脑节段（右和左新皮质、右和左小脑、小脑和脑干）的湿重、DNA含量和蛋白质含量进行定量。出生前或出生后暴露于乙醇的大脑总重量减少了12%。只有新皮质和小脑的重量显着降低了产前或产后治疗。有趣的是，出生后的乙醇暴露增加的体重的campi。特定脑段的DNA和蛋白质含量受到乙醇暴露的影响，但所有结构没有受到类似的影响。最大的影响是在新皮层;这两项措施降低出生前或出生后的乙醇暴露。小脑的DNA和蛋白质含量也受到出生前或出生后乙醇暴露的影响。相比之下，虽然海马体中的DNA含量不受产前暴露的影响，但出生后乙醇暴露显着增加。因此，乙醇诱导的小头畸形以及DNA和蛋白质含量的差异是由细胞数量和神经元体积的变化引起的。然而，这些影响，不同的中枢神经系统结构中的时间依赖性的方式表明，乙醇的主要，但不是唯一的目标是增殖细胞。
A hallmark of fetal alcohol syndrome is microencephaly. Microencephaly can result from either a reduction in the number of cells, in the amount of neuropil, or both. Inasmuch as CNS structures develop along different time lines, the window of vulnerability to ethanol for each structure may differ. Therefore, the growth of the brain after prenatal exposure to ethanol was compared with that following postnatal ethanol exposure. Rats were exposed to ethanol prenatally (between gestational days 6 and 21 via oral consumption by the pregnant dam) or postnatally (between postnatal days 4 and 12 via intubation). The mean of the daily peak blood ethanol concentration was 146 ± 16 and 127 ± 18 mg/dl for the rats exposed to ethanol pre- or postnatally, respectively. Pair-fed control rats were generated for both the pre- and postnatal conditions. Wet weights, DNA content, and protein content were quantified for 6 brain segments (right and left neocortices, right and left hippocampi, the cerebella, and brainstems) of 21-day-old rats. The total weight of the brain was reduced 12% by pre- or postnatal exposure to ethanol. Only the weights of the neocortex and the cerebellum were significantly reduced by pre- or postnatal treatment. Interestingly, postnatal ethanol exposure increased the weight of the hippocampi. The DNA and protein contents of specific brain segments were affected by ethanol exposure, but all structures were not affected similarly. The greatest effect was on the neocortex; both measures decreased following pre- or postnatal ethanol exposure. The DNA and protein contents of the cerebellum were also affected by pre- or postnatal ethanol exposure. In contrast, while DNA content in the hippocampus was unaffected by prenatal exposure, it was significantly increased by postnatal ethanol exposure. Thus, ethanol-induced microcephaly and differences in DNA and protein content result from changes in cell number and in neuropil volume. These effects, however, differ among CNS structures in a time-dependent manner suggesting that the primary, but not sole target of ethanol is the proliferating cell.
登录
查看更多内容
产后早期酒精暴露后神经元的差异性损失。
DOI：
10.1002/tera.1420400205
发表时间：
1989
期刊：
Teratology
影响因子：
--
作者：
Pierce,DR;Goodlett,CR;West,JR
通讯作者：
West,JR
新生大鼠的性别二态性行为和大脑不对称：产前酒精暴露的影响。
DOI：
10.1016/0165-3806(89)90291-5
发表时间：
1989
期刊：
Brain research. Developmental brain research
影响因子：
--
作者：
Zimmerberg,B;Reuter,JM
通讯作者：
Reuter,JM
妊娠晚期乙醇暴露对大鼠海马和齿状回神经元数量的影响。
DOI：
10.1111/j.1530-0277.1986.tb05070.x
发表时间：
1986
期刊：
Alcoholism, clinical and experimental research
影响因子：
--
作者：
West,JR;Hamre,KM;Cassell,MD
通讯作者：
Cassell,MD
DOI：
10.1111/j.1530-0277.1990.tb00455.x
发表时间：
1990-02-01
期刊：
ALCOHOL-CLINICAL AND EXPERIMENTAL RESEARCH
影响因子：
--
作者：
BONTHIUS, DJ;WEST, JR
通讯作者：
WEST, JR
DOI：
10.1111/j.1530-0277.1994.tb00024.x
发表时间：
1994-04-01
影响因子：
3.2
作者：
MILLER, MW;ALRABIAI, S
通讯作者：
ALRABIAI, S