Distribution and molecular characterization of Wolbachia endosymbionts and filarial nematodes in Maryland populations of the lone star tick (Amblyomma americanum).
Distribution and molecular characterization of Wolbachia endosymbionts and filarial nematodes in Maryland populations of the lone star tick (Amblyomma americanum).
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DOI：
10.1111/j.1574-6941.2011.01089.x
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发表时间：
2011-07
影响因子：
4.2
通讯作者：
Rasgon JL
中科院分区：
文献类型：
--
作者：
Zhang X;Norris DE;Rasgon JL
孤独的星星蜱Amblyomma americanum是多种内共生细菌的宿主。我们发现了一种新的Wolbachia共生体感染A。美国。多位点序列分型将内共生体遗传地置于日益多样化的F超群中。我们检测了来自16个马里兰州人群的共计1031只蜱（119只雌性，78只雄性和834只蜱，共89个池）的感染情况。在自然人群中，女性的感染频率约为5%，女性的感染频率<2%（最低感染率）;在男性中未检测到感染。仅在马里兰州南部观察到感染人群，这表明沃尔巴克氏体目前可能正在入侵马里兰州A。美洲种群由于先前已在丝虫中检测到F超群沃尔巴克氏体，因此通过PCR测定蜱样品中的线虫。在沃尔巴克氏体阳性和阴性蜱样本中分别检测到70%和9%的丝虫线虫。虽然线虫在沃尔巴克氏体阳性蜱样本中更常见，但缺乏严格的感染一致性（沃尔巴克氏体阳性，线虫阴性和沃尔巴克氏体阴性，线虫阳性蜱）表明蜱中沃尔巴克氏体的流行不是由于线虫感染。支持这一假设，系统发育分析表明，线虫可能是一个新的物种属Acanthocheilonema，这是以前已被证明是沃尔巴克氏体免费。
The lone star tick Amblyomma americanum is host to a wide diversity of endosymbiotic bacteria. We identified a novel Wolbachia symbiont infecting A. americanum. Multilocus sequence typing phylogenetically placed the endosymbiont in the increasingly diverse F supergroup. We assayed a total of 1031 ticks (119 females, 78 males and 834 nymphs in 89 pools) from 16 Maryland populations for infection. Infection frequencies in the natural populations were approximately 5% in females and <2% (minimum infection rate) in nymphs; infection was not detected in males. Infected populations were only observed in southern Maryland, suggesting the possibility that Wolbachia is currently invading Maryland A. americanum populations. Because F supergroup Wolbachia have been detected previously in filarial nematodes, tick samples were assayed for nematodes by PCR. Filarial nematodes were detected in 70% and 9% of Wolbachia-positive and Wolbachia-negative tick samples, respectively. While nematodes were more common in Wolbachia-positive tick samples, the lack of a strict infection concordance (Wolbachia-positive, nematode-negative and Wolbachia-negative, nematode-positive ticks) suggests that Wolbachia prevalence in ticks is not due to nematode infection. Supporting this hypothesis, phylogenetic analysis indicated that the nematodes were likely a novel species within the genus Acanthocheilonema, which has been previously shown to be Wolbachia-free.
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DOI：
10.1098/rspb.2000.1139
发表时间：
2000-07-07
影响因子：
4.7
作者：
Werren, JH;Windsor, DM
通讯作者：
Windsor, DM
影响因子：
2.6
作者：
Baldo, Laura;Prendini, Lorenzo;Werren, John H.
通讯作者：
Werren, John H.
影响因子：
4.4
作者：
Benson, MJ;Gawronski, JD;Benson, DR
通讯作者：
Benson, DR
影响因子：
2.6
作者：
Turelli, M;Hoffmann, AA
通讯作者：
Hoffmann, AA
影响因子：
2
作者：
Covacin, Catherine;Barker, Stephen C.
通讯作者：
Barker, Stephen C.