Heterologous Expression of the Formicamycin Biosynthetic Gene Cluster Unveils Glycosylated Fasamycin Congeners.
Heterologous Expression of the Formicamycin Biosynthetic Gene Cluster Unveils Glycosylated Fasamycin Congeners.
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DOI：
10.1021/acs.jnatprod.3c00052
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发表时间：
2023-07-28
影响因子：
5.1
通讯作者：
Wilkinson, Barrie
中科院分区：
文献类型：
--
作者：
McDonald, Hannah P.;Alford, Abigail;Devine, Rebecca;Hems, Edward S.;Nepogodiev, Sergey A.;Arnold, Corinne J.;Rejzek, Martin;Stanley-Smith, Anna;Holmes, Neil A.;Hutchings, Matthew I.;Wilkinson, Barrie
福米霉素及其生物合成中间体法霉素是由甲酸链霉菌KY5通过生物合成基因簇编码的途径产生的聚酮抗生素。本研究对天蓝色链霉菌M1146和黑曲霉多孢菌Δery异源表达该生物合成基因簇的能力进行了研究。这导致鉴定了8种新的糖基化法霉素，其在不同的酚基团处被单糖（葡萄糖、半乳糖或葡萄糖醛酸）或由近端己糖（葡萄糖或半乳糖）组成的二糖修饰，具有末端戊糖（阿拉伯糖）部分。与相应的糖苷配基相反，最小抑制筛选试验显示这些糖基化同系物缺乏抗菌活性。
Formicamycins and their biosynthetic intermediates the fasamycins are polyketide antibiotics produced by Streptomyces formicae KY5 from a pathway encoded by the for biosynthetic gene cluster. In this work the ability of Streptomyces coelicolor M1146 and the ability of Saccharopolyspora erythraea Δery to heterologously express the for biosynthetic gene cluster were assessed. This led to the identification of eight new glycosylated fasamycins modified at different phenolic groups with either a monosaccharide (glucose, galactose, or glucuronic acid) or a disaccharide comprised of a proximal hexose (either glucose or galactose), with a terminal pentose (arabinose) moiety. In contrast to the respective aglycones, minimal inhibitory screening assays showed these glycosylated congeners lacked antibacterial activity.
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影响因子：
8.4
作者：
Qin Z;Munnoch JT;Devine R;Holmes NA;Seipke RF;Wilkinson KA;Wilkinson B;Hutchings MI
通讯作者：
Hutchings MI
影响因子：
3.2
作者：
Schell, Ursula;Haydock, Stephen F.;Gaisser, Sabine
通讯作者：
Gaisser, Sabine
影响因子：
15
作者：
Feng, Zhiyang;Chakraborty, Debjani;Dewell, Scott B.;Reddy, Boojala Vijay B.;Brady, Sean F.
通讯作者：
Brady, Sean F.
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL
影响因子：
3.2
作者：
Gregory, MA;Till, R;Smith, MCM
通讯作者：
Smith, MCM