A Rapid Caspase-11 Response Induced by IFNγ Priming Is Independent of Guanylate Binding Proteins.
A Rapid Caspase-11 Response Induced by IFNγ Priming Is Independent of Guanylate Binding Proteins.
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干扰素γ启动诱导的Caspase-11快速反应不依赖鸟苷结合蛋白。
DOI：
10.1016/j.isci.2020.101612
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发表时间：
2020-10-23
期刊：
影响因子：
5.8
通讯作者：
Monack DM
中科院分区：
文献类型：
--
作者：
Brubaker SW;Brewer SM;Massis LM;Napier BA;Monack DM
在哺乳动物细胞中，炎性半胱天冬酶通过结合脂多糖（LPS）来检测革兰氏阴性细菌的入侵。小鼠胱天蛋白酶-11结合胞质LPS，刺激焦萎细胞死亡，并驱动脓毒症发病机制。细胞外引发因子增强caspase-11依赖性焦亡。在此，我们比较了引发剂，并证明IFNγ引发激发了最快速和放大的巨噬细胞对胞质LPS的反应。先前的研究表明，干扰素诱导的caspase-11和鸟苷酸结合蛋白（GBP）的表达是解释引发对胞质LPS传感的影响的因果事件。我们证明这些事件不能完全解释IFNγ治疗引起的应答增加。事实上，当巨噬细胞稳定表达半胱天冬酶-11时，IFNγ引发引起响应于胞质LPS的更高的焦亡水平。在缺乏3号染色体编码的GBP的巨噬细胞中，与其他引发剂相比，IFNγ引发增强了对胞质LPS的反应。这些结果表明存在一种未知的caspase-11依赖性细胞凋亡调节因子，其活性被IFNγ上调。IFNγ引发引起对胞质LPS的最快速和放大的反应增强的IFNγ触发的反应与CASP 11表达是分开的增强的IFN γ触发的反应与3号染色体上编码的GBP无关我们提出了一种未知的IFNγ诱导的CASP 11依赖性细胞凋亡的调节剂存在免疫学;细胞生物学
In mammalian cells, inflammatory caspases detect Gram-negative bacterial invasion by binding lipopolysaccharides (LPS). Murine caspase-11 binds cytosolic LPS, stimulates pyroptotic cell death, and drives sepsis pathogenesis. Extracellular priming factors enhance caspase-11-dependent pyroptosis. Herein we compare priming agents and demonstrate that IFNγ priming elicits the most rapid and amplified macrophage response to cytosolic LPS. Previous studies indicate that IFN-induced expression of caspase-11 and guanylate binding proteins (GBPs) are causal events explaining the effects of priming on cytosolic LPS sensing. We demonstrate that these events cannot fully account for the increased response triggered by IFNγ treatment. Indeed, IFNγ priming elicits higher pyroptosis levels in response to cytosolic LPS when macrophages stably express caspase-11. In macrophages lacking GBPs encoded on chromosome 3, IFNγ priming enhanced pyroptosis in response to cytosolic LPS as compared with other priming agents. These results suggest an unknown regulator of caspase-11-dependent pyroptosis exists, whose activity is upregulated by IFNγ. IFNγ priming elicits the most rapid and amplified response to cytosolic LPS The enhanced IFNγ-triggered response is separable from CASP11 expression The enhanced IFNγ-triggered response is independent of GBPs encoded on chromosome 3 We propose an unknown IFNγ-induced regulator of CASP11-dependent pyroptosis exists Immunology; Cell Biology
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影响因子：
6.4
作者：
Finethy R;Luoma S;Orench-Rivera N;Feeley EM;Haldar AK;Yamamoto M;Kanneganti TD;Kuehn MJ;Coers J
通讯作者：
Coers J
影响因子：
29.7
作者：
Brubaker SW;Bonham KS;Zanoni I;Kagan JC
通讯作者：
Kagan JC
DOI：
10.1073/pnas.1607769113
发表时间：
2016-07-12
影响因子：
11.1
作者：
Aglietti, Robin A.;Estevez, Alberto;Dueber, Erin C.
通讯作者：
Dueber, Erin C.
DOI：
10.1126/science.1240988
发表时间：
2013-09-13
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hagar JA;Powell DA;Aachoui Y;Ernst RK;Miao EA
通讯作者：
Miao EA
影响因子：
64.5
作者：
Man, Si Ming;Karki, Rajendra;Sasai, Miwa;Place, David E.;Kesavardhana, Sannula;Temirov, Jamshid;Frase, Sharon;Zhu, Qifan;Malireddi, R. K. Subbarao;Kuriakose, Teneema;Peters, Jennifer L.;Neale, Geoffrey;Brown, Scott A.;Yamamoto, Masahiro;Kanneganti, Thirumala-Devi
通讯作者：
Kanneganti, Thirumala-Devi