Embedded Human Periodontal Ligament Stem Cells Spheroids Enhance Cementogenic Differentiation via Plasminogen Activator Inhibitor 1.
Embedded Human Periodontal Ligament Stem Cells Spheroids Enhance Cementogenic Differentiation via Plasminogen Activator Inhibitor 1.
复制标题
DOI：
10.3390/ijms23042340
复制
发表时间：
2022-02-20
影响因子：
5.6
通讯作者：
Ohno J
中科院分区：
文献类型：
--
作者：
Yasunaga M;Ishikawa H;Tamaoki S;Maeda H;Ohno J
球体比经典的二维（2D）单层培养物更准确地再现了体内发现的组织结构。我们培养的人牙周膜干细胞（HPLSCs）的球状体包埋在胶原凝胶，以检查是否可以增强其牙骨质分化与重组人纤溶酶原激活物抑制剂-1（rhPAI-1）的治疗。与对照组和成骨诱导培养基组相比，rhPAI-1处理3周的2D单层HPLSCs中牙骨质蛋白1（CEMP 1）和牙骨质附着蛋白（CAP）的表达上调，这是已建立的成牙骨质细胞标志物。在包埋的HPLSC球状体中，rhPAI-1处理诱导球状体和胶原性细胞外基质（ECM）之间的相互作用，表明在三维（3D）培养后72小时，解聚的HPLSC迁移并扩散到周围的ECM中。Western blot和免疫细胞化学分析显示，与所有2D单层HPLSC组和3D球体组相比，rhPAI-1处理的包埋HPLSC球体中CEMP 1的表达水平显著上调。因此，3D胶原包埋球体培养结合rhPAI-1处理可能有助于促进HPLSC的牙骨质分化。
Spheroids reproduce the tissue structure that is found in vivo more accurately than classic two-dimensional (2D) monolayer cultures. We cultured human periodontal ligament stem cells (HPLSCs) as spheroids that were embedded in collagen gel to examine whether their cementogenic differentiation could be enhanced by treatment with recombinant human plasminogen activator inhibitor-1 (rhPAI-1). The upregulated expression of cementum protein 1 (CEMP1) and cementum attachment protein (CAP), established cementoblast markers, was observed in the 2D monolayer HPLSCs that were treated with rhPAI-1 for 3 weeks compared with that in the control and osteogenic-induction medium groups. In the embedded HPLSC spheroids, rhPAI-1 treatment induced interplay between the spheroids and collagenous extracellular matrix (ECM), indicating that disaggregated HPLSCs migrated and spread into the surrounding ECM 72 h after three-dimensional (3D) culture. Western blot and immunocytochemistry analyses showed that the CEMP1 expression levels were significantly upregulated in the rhPAI-1-treated embedded HPLSC spheroids compared with all the 2D monolayer HPLSCs groups and the 3D spheroid groups. Therefore, 3D collagen-embedded spheroid culture in combination with rhPAI-1 treatment may be useful for facilitating cementogenic differentiation of HPLSCs.
登录
查看更多内容
影响因子：
7.6
作者：
Arzate, H;Jiménez-García, LF;Pitaru, S
通讯作者：
Pitaru, S
影响因子：
4.1
作者：
Nordstrom, S. M.;Carleton, S. M.;Vaughan, D. E.
通讯作者：
Vaughan, D. E.
影响因子：
64.5
作者：
Takahashi, Kazutoshi;Yamanaka, Shinya
通讯作者：
Yamanaka, Shinya
DOI：
10.1007/978-1-4419-1050-9_5
发表时间：
2010-01-01
期刊：
OSTEOIMMUNOLOGY: INTERACTIONS OF THE IMMUNE AND SKELETAL SYSTEMS II
影响因子：
--
作者：
Komori, Toshihisa
通讯作者：
Komori, Toshihisa
影响因子：
3.1
作者：
Huang, G. -S.;Tseng, C. -S.;Hsu, S. -h.
通讯作者：
Hsu, S. -h.