Ascorbate oxidase electrochemical biosensor based on the biocompatible poly(3, 4-ethylenedioxythiophene) matrices for agricultural application in crops

Ascorbate oxidase electrochemical biosensor based on the biocompatible poly(3, 4-ethylenedioxythiophene) matrices for agricultural application in crops
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基于生物相容性聚(3, 4-乙烯二氧噻吩)基质的抗坏血酸氧化酶电化学生物传感器,用于农作物农业应用

DOI:
10.1016/j.cclet.2011.11.010
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发表时间:
2012-02
影响因子:
9.1
通讯作者:
Xu, Jing Kun
Xu, Jing Kun
中科院分区:
化学1区
文献类型:
--
作者:
Wen, Yang Ping;Lu, Li Min;Li, Dong;Liu, Ming;He, Hao Hua;Xu, Jing Kun

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利用基于生物相容性聚(3, 4-乙撑二氧噻吩)(PEDOT)基质的抗坏血酸氧化酶(AO)电化学生物传感器成功测定了农作物中的维生素C(VC),该基质可通过离子液体微乳液中的一步电沉积技术轻松制备。所制备的生物传感器对VC的氧化表现出优异的生物电催化性能,线性范围宽,检测限低,响应时间快,操作和储存稳定性好,蔬菜作物中VC测定的良好结果表明,该生物传感器将在不久的将来成为作物生理生化研究的良好候选者。
The vitamin C (VC) in crops was successfully determined using ascorbate oxidase (AO) electrochemical biosensor based on the biocompatible poly(3, 4-ethylenedioxythiophene) (PEDOT) matrices, which was easily prepared by one-step electrodeposition technique in ionic liquid microemulsions. The fabricated biosensor displayed excellent bioelectrocatalytic performance to the oxidation of VC, wide linear range, low detection limit, fast response time, good operational and storage stability, the good results of the determination of VC in vegetable crops indicated that the fabricated biosensor will be a good candidate for the physiological and biochemical studies of crops in near future.
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