Swallowing-like activity elicited in vitro in neonatal rat organ attached brainstem block preparation
Swallowing-like activity elicited in vitro in neonatal rat organ attached brainstem block preparation
复制标题
新生大鼠器官附着脑干块制剂在体外引发吞咽样活动
DOI：
10.1016/s0006-8993(02)03339-5
复制
发表时间：
2002
期刊：
影响因子：
2.9
通讯作者：
T. Matsuya
中科院分区：
文献类型：
--
作者：
M. Kogo;T. Yamanishi;H. Koizumi;T. Matsuya
本研究的目的是在体外新生大鼠脑干标本中诱导吞咽，并分析回路。在新生大鼠（生后0- 3d）的器官附着脑干标本上，将GABA A受体拮抗剂（Bicuculine methiodide，BIC）注入孤束核（NTS）内，可观察到下颌闭合运动、腭上举和舌下垂样运动，随后出现舌尖上举和喉前移（气管关闭）。NTS被认为是哺乳动物吞咽模式产生的关键位点。在记录室中应用BIC（10 μM）后，电刺激NTS或迷走传入神经（X）引发相同的器官运动。这种运动引起呼吸活动的暂时抑制，同时从第四颈腹神经（C4）记录。我们还能够在整个器官（从唇到胃，中线完整）制备物中引发这种活性，其口腔充满染料（pontamine天蓝3 mM，50 μl），使用三种类型的刺激中的每一种。食管，这是从来没有被自发呼吸运动染色，染色后，只有实验刺激。我们的结论是，引起的活动是吞咽样活动和吞咽模式产生的最小电路存在于该制剂中。
The purpose of this study was to induce swallowing in an in vitro neonatal rat brainstem preparation and to analyze the circuit. When we applied GABAAreceptor antagonist (bicuculine methiodide, BIC) into the the nucleus tractus solitarius (NTS) in the organ attached brainstem preparation of neonatal (0–3 days after birth) rats, jaw closing movement, palatal lifting, and tongue peristalsis-like movement were seen, subsequent to elevation of the tip of the tongue and anterior movement of the larynx (closure of the trachea). The NTS has been proposed to be a critical locus for swallowing pattern generation in mammals. Electrical stimulation into the NTS or the vagal afferent nerve (X) following an application of BIC (10 μM) to the recording chamber initiated the same organ movement. This movement caused temporary inhibition of respiratory activity that was simultaneously recorded from the fourth cervical ventral nerve (C4). We were also able to elicit this activity in a whole organ (from lip to stomach, midline intact) preparation, whose oral cavity was filled with dye (pontamine sky-blue 3 mM, 50 μl), using each of the three types of stimulation. The esophagus, which was never stained by spontaneous respiratory movements, was stained only after the experimental stimulation. We concluded that the activity elicited was swallowing-like activity and the smallest circuit for swallowing pattern generation exists in this preparation.
影响因子：
56.9
作者：
SMITH, JC;ELLENBERGER, HH;FELDMAN, JL
通讯作者：
FELDMAN, JL
迷走神经刺激诱导体外新生大鼠呼气延长。
DOI：
10.1152/jappl.1997.83.5.1607
发表时间：
1997
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
--
作者：
Mellen,NM;Feldman,JL
通讯作者：
Feldman,JL