In ovo supplementation of chitooligosaccharide and chlorella polysaccharide affects cecal microbial community, metabolic pathways, and fermentation metabolites in broiler chickens.
In ovo supplementation of chitooligosaccharide and chlorella polysaccharide affects cecal microbial community, metabolic pathways, and fermentation metabolites in broiler chickens.
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DOI：
10.1016/j.psj.2020.06.061
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发表时间：
2020-10
期刊：
影响因子：
4.4
通讯作者：
Jha R
中科院分区：
文献类型：
--
作者：
Zhang J;Cai K;Mishra R;Jha R
壳寡糖（COS）和小球藻多糖（CPS）在家禽业中被用作饲料添加剂，以提高生长性能和免疫力。然而，在早期营养中使用这些益生元对鸡肠道健康的益处尚不清楚。本研究评估了蛋饲COS和CPS对鸡盲肠微生物群、代谢途径和发酵代谢物的影响。将240个受精卵分为6组（n = 4, 10个/重复）：1)不注射对照组，2)生理盐水对照组，3)COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, 6)注射CPS 20 mg。卵孵化第12.5天，将试验底物注射到不同处理的卵的羊膜囊内。雏鸡在标准饲养条件下饲养21 D。第3天和第21天采集盲肠食糜，采用散弹枪宏基因组测序法测定菌群，采用气相色谱法测定短链脂肪酸。第3天各组盲肠微生物组成差异无统计学意义（P < 0.05），第21天差异有统计学意义（P < 0.05）。在菌种水平上，COS组利用多糖的约氏乳杆菌、coprocola拟杆菌和salanitronis拟杆菌的相对丰度较高，而一些条件致病菌的相对丰度低于CPS组和对照组。在功能水平上，COS组糖异生、l -异亮氨酸降解、l -组氨酸生物合成和脂肪酸生物合成途径丰富。COS组丙酸含量显著高于对照组（P < 0.05）。通过构建COS与其他因素的相关性网络，阐明COS在鸡早期营养中的潜在作用机制。综上所述，蛋鸡接种COS 5 mg对盲肠菌群、代谢途径和丙酸均有积极影响，可用于蛋鸡饲养中调节肠道健康。
The chitooligosaccharide (COS) and chlorella polysaccharide (CPS) have been used as feed supplements in the poultry industry for improving growth performance and immunity. However, the benefits of these prebiotics on the gut health of chickens when used in early nutrition are unknown. This study evaluated the effects of in ovo feeding of COS and CPS on the cecal microbiome, metabolic pathways, and fermentation metabolites of chickens. A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection. On day 12.5 of egg incubation, test substrate was injected into the amniotic sac of eggs in respective treatments. The hatched chicks were raised for 21 D under standard husbandry practices. On day 3 and 21, cecal digesta were collected to determine microbiota by shotgun metagenomic sequencing and short-chain fatty acids by gas chromatography. The cecal microbial composition was not different (P > 0.05) among the treatment groups on day 3 but was different (P < 0.05) on day 21. At the species level, the polysaccharide-utilizing bacteria including Lactobacillus johnsonii, Bacteroides coprocola, and Bacteroides salanitronis were higher in the COS group, whereas the relative abundance of some opportunistic pathogenic bacteria were lower than those in the CPS and control groups. At the functional level, the pathways of gluconeogenesis, L-isoleucine degradation, L-histidine biosynthesis, and fatty acid biosynthesis were enriched in the COS group. In addition, propionic acid content was higher (P < 0.05) in the COS group. A network based on the correlation between the COS and other factors was constructed to illuminate the potential action mechanism of the COS in chicken early nutrition. In conclusion, in ovo inoculation of COS 5 mg showed positive effects on the cecal microbiota, metabolic pathways, and propionic acid, thus can be used as in ovo feeding to modulate the gut health of chickens.
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影响因子：
3.6
作者：
Jha, R.;Leterme, P.
通讯作者：
Leterme, P.
影响因子：
5.2
作者：
Laokuldilok, Thunnop;Potivas, Thipwimon;Regenstein, Joe M.
通讯作者：
Regenstein, Joe M.
影响因子：
0.5
作者：
Jiao, Yang;Jha, Rajesh;Kim, In Ho
通讯作者：
Kim, In Ho
DOI：
10.1017/s1751731115000919
发表时间：
2015-09
期刊：
Animal : an international journal of animal bioscience
影响因子：
--
作者：
Jha R;Berrocoso JD
通讯作者：
Berrocoso JD
影响因子：
2.4
作者：
Meimandipour, A.;Shuhaimi, M.;Yazid, A. M.
通讯作者：
Yazid, A. M.