Circadian dynamics of the teleost skin immune-microbiome interface
Circadian dynamics of the teleost skin immune-microbiome interface
复制标题
硬骨鱼皮肤免疫-微生物组界面的昼夜动态
DOI：
10.1101/2021.01.29.428758
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Ellison A
中科院分区：
文献类型：
--
作者：
Ellison A
背景宿主免疫活动及其微生物组的昼夜节律可能对健康和抗病能力至关重要。然而，整合时间治疗方法来减轻管理动物的疾病尚未实现。在水产养殖中，光操纵通常用于促进生长和控制繁殖，但可能对动物健康产生未知的负面影响。传染病是可持续水产养殖的主要障碍，迫切需要了解鱼类免疫的昼夜动态以及与微生物组的串扰。结果在这里，我们以虹鳟鱼（Oncorhynchus mykiss）为模型，结合16S rRNA宏条形码、宏基因组测序和直接mRNA定量方法，同时表征皮肤时钟和免疫基因表达的昼夜动态以及皮肤微生物群的日常变化。我们展示了鱼皮肤免疫表达和微生物组的日常节律，这些节律受到光周期和寄生虱子感染的调节。我们确定了宿主时钟和免疫基因谱与微生物组成的假定关联。我们的结果表明，昼夜节律扰动会改变免疫和微生物群活动的幅度和时间，对鱼类健康有害。结论我们发现的实质性昼夜节律动态和鱼类宿主表达-微生物组关系为研究时间疗法在水产养殖中的效用提供了宝贵的基础，并且更广泛地有助于我们了解微生物组在脊椎动物昼夜节律健康中的作用。视频摘要
BackgroundCircadian rhythms of host immune activity and their microbiomes are likely pivotal to health and disease resistance. The integration of chronotherapeutic approaches to disease mitigation in managed animals, however, is yet to be realised. In aquaculture, light manipulation is commonly used to enhance growth and control reproduction but may have unknown negative consequences for animal health. Infectious diseases are a major barrier to sustainable aquaculture and understanding the circadian dynamics of fish immunity and crosstalk with the microbiome is urgently needed.ResultsHere, using rainbow trout (Oncorhynchus mykiss) as a model, we combine 16S rRNA metabarcoding, metagenomic sequencing and direct mRNA quantification methods to simultaneously characterise the circadian dynamics of skin clock and immune gene expression, and daily changes of skin microbiota. We demonstrate daily rhythms in fish skin immune expression and microbiomes, which are modulated by photoperiod and parasitic lice infection. We identify putative associations of host clock and immune gene profiles with microbial composition. Our results suggest circadian perturbation, that shifts the magnitude and timing of immune and microbiota activity, is detrimental to fish health.ConclusionsThe substantial circadian dynamics and fish host expression-microbiome relationships we find represent a valuable foundation for investigating the utility of chronotherapies in aquaculture, and more broadly contributes to our understanding of the role of microbiomes in circadian health of vertebrates.Video Abstract
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
30.3
作者：
Leone V;Gibbons SM;Martinez K;Hutchison AL;Huang EY;Cham CM;Pierre JF;Heneghan AF;Nadimpalli A;Hubert N;Zale E;Wang Y;Huang Y;Theriault B;Dinner AR;Musch MW;Kudsk KA;Prendergast BJ;Gilbert JA;Chang EB
通讯作者：
Chang EB
DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1038/nri3386
发表时间：
2013-03
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
通讯作者：
--
影响因子：
2.9
作者：
Fumio Takizawa;J. M. Dijkstra;P. Kotterba;T. Korytář;H. Kock;B. Köllner;B. Jaureguiberry;T. Nakanishi;U. Fischer
通讯作者：
Fumio Takizawa;J. M. Dijkstra;P. Kotterba;T. Korytář;H. Kock;B. Köllner;B. Jaureguiberry;T. Nakanishi;U. Fischer