Comparative Transcriptomics Reveals Jasmonic Acid-Associated Metabolism Related to Cotton Fiber Initiation.
Comparative Transcriptomics Reveals Jasmonic Acid-Associated Metabolism Related to Cotton Fiber Initiation.
复制标题
比较转录组学揭示与棉纤维起始相关的茉莉酸相关代谢
DOI：
10.1371/journal.pone.0129854
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Guo W
中科院分区：
文献类型：
--
作者：
Wang L;Zhu Y;Hu W;Zhang X;Cai C;Guo W
突变体和基因表达模式的分析为研究植物纤维发育关键阶段的基因提供了有力的手段。本研究以无绒-无绒XinWX和无绒-无绒XinFLM为材料，利用单基因座差异筛选差异表达基因。扫描电子显微镜观察表明，花后0天（DPA），新FLM纤维发生。利用寡核苷酸微阵列对三个起始发育阶段（-1，0，1 DPA）的品系进行纤维转录谱分析。环内和线之间的差异表达的基因进行了比较，功能分类和富集分析表明，在纤维起始过程中的基因表达模式与激素代谢，转录因子调控，脂质转运，和天冬酰胺生物合成过程中，如前所述。此外，在茉莉酸生物合成中起作用的丙二烯氧化物环化酶（AOC）家族的四个成员在纤维起始中平行上调，特别是在-1 DPA时，与棉绒绒毛TM-1中的其他组织和器官相比。真实的时间定量PCR（RT-qPCR）分析表明，在-1 DPA处理下，无绒绒材料的AOC表达量高于有绒和有绒绒材料，茉莉酸（JA）处理提高了AOC的转录水平。对XinWX和XinFLM之间JA生物合成相关基因的表达分析表明，它们在无绒-无绒突变体的纤维起始过程中上调。综上所述，茉莉酸相关代谢与棉纤维起始有关。AOCs表达的平行上调可能对正常的纤维起始发育很重要，而AOCs的过量产生可能会破坏正常的纤维发育。
Analysis of mutants and gene expression patterns provides a powerful approach for investigating genes involved in key stages of plant fiber development. In this study, lintless-fuzzless XinWX and linted-fuzzless XinFLM with a single genetic locus difference for lint were used to identify differentially expressed genes. Scanning electron microscopy showed fiber initiation in XinFLM at 0 days post anthesis (DPA). Fiber transcriptional profiling of the lines at three initiation developmental stages (-1, 0, 1 DPA) was performed using an oligonucleotide microarray. Loop comparisons of the differentially expressed genes within and between the lines was carried out, and functional classification and enrichment analysis showed that gene expression patterns during fiber initiation were heavily associated with hormone metabolism, transcription factor regulation, lipid transport, and asparagine biosynthetic processes, as previously reported. Further, four members of the allene-oxide cyclase (AOC) family that function in jasmonate biosynthesis were parallel up-regulation in fiber initiation, especially at -1 DPA, compared to other tissues and organs in linted-fuzzed TM-1. Real time-quantitative PCR (RT-qPCR) analysis in different fiber mutant lines revealed that AOCs were up-regulated higher at -1 DPA in lintless-fuzzless than that in linted-fuzzless and linted-fuzzed materials, and transcription of the AOCs was increased under jasmonic acid (JA) treatment. Expression analysis of JA biosynthesis-associated genes between XinWX and XinFLM showed that they were up-regulated during fiber initiation in the fuzzless-lintless mutant. Taken together, jasmonic acid-associated metabolism was related to cotton fiber initiation. Parallel up-regulation of AOCs expression may be important for normal fiber initiation development, while overproduction of AOCs might disrupt normal fiber development.
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影响因子：
3.3
作者：
Liu, Kang;Han, Meiling;Zhang, Tianzhen
通讯作者：
Zhang, Tianzhen
DOI：
10.1073/pnas.93.23.12768
发表时间：
1996-11-12
影响因子：
11.1
作者：
John, ME;Keller, G
通讯作者：
Keller, G
影响因子：
7.4
作者：
Howe, GA;Lee, GI;DeRocher, AE
通讯作者：
DeRocher, AE
影响因子：
3.7
作者：
Feng, JX;Ji, SJ;Zhu, YX
通讯作者：
Zhu, YX
影响因子：
4.3
作者：
Lee, Joohyun;Burns, Teresa H.;Allen, Randy D.
通讯作者：
Allen, Randy D.