Three forms of RPTP‐β are differentially expressed during gliogenesis in the developing rat brain and during glial cell differentiation in culture
Three forms of RPTP‐β are differentially expressed during gliogenesis in the developing rat brain and during glial cell differentiation in culture
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三种形式的 RPTP-β 在发育中的大鼠大脑的胶质生成过程中和培养物中的胶质细胞分化过程中存在差异表达
DOI：
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发表时间：
1996
影响因子：
4.2
通讯作者：
J. Musacchio
中科院分区：
文献类型：
--
作者：
P. Canoll;S. Petanceska;J. Schlessinger;J. Musacchio
原位杂交和北方分析表明，RPTP-β的三种剪接变体在发育中的大脑中具有不同的时空表达模式。编码具有不同胞外结构域的跨膜蛋白酪氨酸磷酸酶的9.5-kb和6.4-kb转录物主要在位于室管膜下区（SVZ）的神经胶质祖细胞中表达。8.4 kb的转录本编码分泌的硫酸软骨素蛋白聚糖（phosphacan），由迁移出SVZ的更成熟的胶质细胞高水平表达。这三种转录本在神经胶质细胞培养物中也有差异表达; O2 A祖细胞表达高水平的9.5和8.4 kb转录本，而1型星形胶质细胞祖细胞主要表达6.4 kb转录本。C6胶质瘤也表达高水平的6.4 kb转录本。用分化剂二丁酰环磷酸腺苷（DBcAMP）处理C6细胞，诱导6.4 kb转录物的减少和8.4 kb转录物的相应增加。在碱性成纤维细胞生长因子（bFGF）和血小板衍生生长因子（PDGF）存在下生长的O2 A细胞保持高度增殖和未分化，并继续表达高水平的RPTP-β。然而，当O2 A细胞在诱导少突胶质细胞分化的条件下生长时，RPTP-β的跨膜形式的表达显著降低，如通过免疫荧光测定的。这些结果表明，RPTP-β表达在神经胶质细胞分化过程中受到调节，并表明不同形式的RPTP-β在大脑发育过程中发挥不同的功能。© 1996 Wiley利斯公司
In situ hybridization and Northern analysis demonstrate that the three splicing variants of RPTP‐β have different spatial and temporal patterns of expression in the developing brain. The 9.5‐kb and 6.4‐kb transcripts, which encode transmembrane protein tyrosine phosphatases with different extracellular domains, are predominantly expressed in glial progenitors located in the subventricular zone (SVZ). The 8.4‐kb transcript, which encodes a secreted chondroitin sulfate proteoglycan (phosphacan), is expressed at high levels by more mature glia that have migrated out of the SVZ. The three transcripts are also differentially expressed in glial cell cultures; O2A progenitors express high levels of the 9.5‐ and 8.4‐kb transcript, whereas type 1 astrocyte progenitors predominantly express the 6.4‐kb transcript. C6 gliomas also express high levels of the 6.4‐kb transcript. Treating C6 cells with the differentiating agent dibutyryl cyclic‐AMP (DBcAMP), induces a decrease in the 6.4‐kb transcript and a corresponding increase in the 8.4‐kb transcript. O2A cells grown in the presence of basic fibroblast growth factor (bFGF) and platelet‐derived growth factor (PDGF) remain highly proliferative and undifferentiated, and continue to express high levels of RPTP‐β. However, when O2A cells are grown in conditions that induce oligodendrocyte differentiation, there is a marked decrease in the expression of the transmembrane forms of RPTP‐β, as determined by immunofluorescence. These results demonstrate that RPTP‐β expression is regulated during glial cell differentiation and suggest that the different forms of RPTP‐β perform distinct functions during brain development. © 1996 Wiley‐Liss, Inc.
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DOI：
10.1073/pnas.92.2.542
发表时间：
1995-01
影响因子：
11.1
作者：
O. Sporns;G. Edelman;K. L. Crossin
通讯作者：
O. Sporns;G. Edelman;K. L. Crossin
DOI：
10.1073/pnas.89.16.7417
发表时间：
1992-08-15
影响因子：
11.1
作者：
KRUEGER, NX;SAITO, H
通讯作者：
SAITO, H
酪氨酸磷酸化蛋白在神经元和神经胶质细胞分化过程中减少。
影响因子：
2.9
作者：
Ingraham,CA;Maness,PF
通讯作者：
Maness,PF
大鼠受体型蛋白酪氨酸磷酸酶-zeta/β的核苷酸序列和分子变异体。
DOI：
10.3109/10425179509030989
发表时间：
1995
期刊：
DNA sequence : the journal of DNA sequencing and mapping
影响因子：
--
作者：
Maurel,P;Meyer-Puttlitz,B;Flad,M;Margolis,RU;Margolis,RK
通讯作者：
Margolis,RK
蛋白质酪氨酸磷酸酶的结构、调节和功能。
DOI：
10.1101/sqb.1991.056.01.032
发表时间：
1991
期刊：
Cold Spring Harbor symposia on quantitative biology
影响因子：
--
作者：
Tonks,NK;Yang,Q;GuidaJr,P
通讯作者：
GuidaJr,P