ATP-binding cassette (ABC) transporter expression and localization in sea urchin development.
ATP-binding cassette (ABC) transporter expression and localization in sea urchin development.
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DOI：
10.1002/dvdy.23786
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发表时间：
2012-06
影响因子：
2.5
通讯作者：
Hamdoun, Amro
中科院分区：
文献类型：
--
作者：
Shipp, Lauren E.;Hamdoun, Amro
ATP-binding cassette (ABC) transporters are membrane proteins that regulate intracellular concentrations of myriad compounds and ions. There are >100 ABC transporter predictions in the Strongylocentrotus purpuratus genome, including 40 annotated ABCB, ABCC, and ABCG “multidrug efflux” transporters. Despite the importance of multidrug transporters for protection and signaling, their expression patterns have not been characterized in deuterostome embryos. Sea urchin embryos expressed 20 ABCB, ABCC, and ABCG transporter genes in the first 58 hours of development, from unfertilized egg to early prism. We quantified transcripts of ABCB1a, ABCB4a, ABCC1, ABCC5a, ABCC9a, and ABCG2b, and found that ABCB1a mRNA was 10–100 times more abundant than other transporter mRNAs. In situ hybridization showed ABCB1a was expressed ubiquitously in embryos, while ABCC5a was restricted to secondary mesenchyme cells and their precursors. Fluorescent protein fusions showed localization of ABCB1a on apical cell surfaces, and ABCC5a on basolateral surfaces. Embryos utilize many ABC transporters with predicted functions in cell signaling, lysosomal and mitochondrial homeostasis, potassium channel regulation, pigmentation, and xenobiotic efflux. Detailed characterization of ABCB1a and ABCC5a revealed that they have different temporal and spatial gene expression profiles and protein localization patterns that correlate to their predicted functions in protection and development, respectively.
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影响因子：
14.9
作者：
Cameron RA;Samanta M;Yuan A;He D;Davidson E
通讯作者：
Davidson E
DOI：
10.1126/science.1168750
发表时间：
2009-03-27
期刊：
Science (New York, N.Y.)
影响因子：
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作者：
Aller SG;Yu J;Ward A;Weng Y;Chittaboina S;Zhuo R;Harrell PM;Trinh YT;Zhang Q;Urbatsch IL;Chang G
通讯作者：
Chang G
DOI：
10.1111/j.1742-4658.2011.08235.x
发表时间：
2011-09
期刊：
The FEBS journal
影响因子：
--
作者：
Chen ZS;Tiwari AK
通讯作者：
Tiwari AK
海胆胚胎III中的口腔宽轴规范。线粒体氧化还原信号通过H2O2的作用。
影响因子：
2.7
作者：
Coffman, James A.;Coluccio, Alison;Planchart, Antonio;Robertson, Anthony J.
通讯作者：
Robertson, Anthony J.
影响因子：
2.7
作者：
Juliano, Celina E.;Voronina, Ekaterina;Wessel, Gary M.
通讯作者：
Wessel, Gary M.