Circulating microbial content in myeloid malignancy patients is associated with disease subtypes and patient outcomes.
Circulating microbial content in myeloid malignancy patients is associated with disease subtypes and patient outcomes.
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DOI：
10.1038/s41467-022-28678-x
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发表时间：
2022-02-24
影响因子：
16.6
通讯作者：
LaFramboise T
中科院分区：
文献类型：
--
作者：
Woerner J;Huang Y;Hutter S;Gurnari C;Sánchez JMH;Wang J;Huang Y;Schnabel D;Aaby M;Xu W;Thorat V;Jiang D;Jha BK;Koyuturk M;Maciejewski JP;Haferlach T;LaFramboise T
虽然最近的工作已经描述了实体瘤中的微生物组，但血液恶性肿瘤中的微生物含量尚未得到很好的表征。在这里，我们分析了1870例骨髓恶性肿瘤患者的血液和骨髓中现有的深层DNA序列数据，沿着健康对照，包括细菌、真菌和病毒含量。经过严格的质量过滤，我们发现了疾病病例中生态失调的证据，以及疾病亚型中不同的微生物特征。我们还发现微生物含量与宿主基因突变和成髓细胞百分比相关。在低危骨髓增生异常综合征患者中，我们提供的证据表明，EB病毒状态将风险分层细化为比当前标准更精确的类别。受这些观察结果的启发，我们构建了机器学习分类器，可以仅基于细菌含量区分疾病亚型。我们的研究强调了循环微生物组与患者结局之间的关联，以及其与疾病亚型的关系。循环微生物组对于血液恶性肿瘤的研究很少。在这里，作者表明血液中特定的微生物组特征与不同类型的骨髓恶性肿瘤和特定的基因突变有关。
Although recent work has described the microbiome in solid tumors, microbial content in hematological malignancies is not well-characterized. Here we analyze existing deep DNA sequence data from the blood and bone marrow of 1870 patients with myeloid malignancies, along with healthy controls, for bacterial, fungal, and viral content. After strict quality filtering, we find evidence for dysbiosis in disease cases, and distinct microbial signatures among disease subtypes. We also find that microbial content is associated with host gene mutations and with myeloblast cell percentages. In patients with low-risk myelodysplastic syndrome, we provide evidence that Epstein-Barr virus status refines risk stratification into more precise categories than the current standard. Motivated by these observations, we construct machine-learning classifiers that can discriminate among disease subtypes based solely on bacterial content. Our study highlights the association between the circulating microbiome and patient outcome, and its relationship with disease subtype. Circulating microbiome has been very little studied for blood malignancies. Here, the authors show specific microbiome signatures in the blood are associated with different types of myeloid malignancies and specific genetic mutations.
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