Differential resuscitative effect of pyruvate and its analogues on VBNC (viable but non-culturable) Salmonella.
Differential resuscitative effect of pyruvate and its analogues on VBNC (viable but non-culturable) Salmonella.
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DOI：
10.1264/jsme2.me12174
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发表时间：
2013
影响因子：
2.2
通讯作者：
Amano F
中科院分区：
文献类型：
--
作者：
Morishige Y;Fujimori K;Amano F
通过向Luria-Bertani（LB）培养基中生长的培养物中加入3 mM H2 O2，生长至对数期的肠道沙门氏菌（SE）的环境分离株迅速丧失可培养性;然而，如果丙酮酸钠以至少0.3mM存在，则一些H2 O2处理的细菌在M9基本培养基中恢复了它们的可培养性。此外，大多数丙酮酸类似物，例如溴丙酮酸或苯丙酮酸，除了α-酮丁酸盐外，没有显示出类似于丙酮酸盐的恢复活性。对丙酮酸复苏机制的进一步分析表明，尽管许多细菌在有或没有丙酮酸的情况下对CTC（5-氰基-2，3-二-（对甲苯基）四唑氯化物）还原显示出呼吸活性，但在丙酮酸存在或不存在的情况下，DNA的生物合成和蛋白质合成是完全不同的，即，丙酮酸盐赋予细胞在复苏过程中将更多的放射性标记掺入前体的能力。这些结果表明，丙酮酸是通过触发大分子如DNA和蛋白质的合成将细菌从不可培养状态带到生长和菌落形成状态的复苏过程中起作用的关键分子之一。
An environmental isolate of Salmonella Enteritidis (SE), grown to the logarithmic phase, rapidly lost culturability by the addition of 3 mM H2O2 to cultures grown in Luria-Bertani (LB) medium; however, some H2O2-treated bacteria regained their culturability in M9 minimal medium, if sodium pyruvate was present at at least 0.3 mM. In addition, most pyruvate analogues, such as bromopyruvate or phenylpyruvate, did not show restoration activity similar to that of pyruvate, except in the case of α-ketobutyrate. Further analysis of the mechanism underlying the resuscitation by pyruvate revealed that although many of the bacteria showed respiratory activity on CTC (5-cyano-2,3-di-(p-tolyl) tetrazolium chloride) reduction with or without pyruvate, the biosynthesis of DNA and protein synthesis were quite different in the presence or absence of pyruvate, i.e., pyruvate endowed the cells with the ability to incorporate much more radio-label into precursors during the resuscitation process. These results suggest that pyruvate is one of the key molecules working in the resuscitation process by taking bacteria from the non-culturable state to the growing and colony-forming state by triggering the synthesis of macromolecules such as DNA and protein.
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影响因子：
4.4
作者：
Gupte, AR;de Rezende, CLE;Joseph, SW
通讯作者：
Joseph, SW
影响因子：
4
作者：
Lleò, MM;Bonato, B;Canepari, P
通讯作者：
Canepari, P
影响因子：
2.4
作者：
CHMIELEWSKI, RAN;FRANK, JF
通讯作者：
FRANK, JF
影响因子：
2
作者：
Little, C. L.;Rhoades, J. R.;Chan, C. -H.
通讯作者：
Chan, C. -H.
影响因子：
2.8
作者：
CALABRESE, JP;BISSONNETTE, GK
通讯作者：
BISSONNETTE, GK