Size and sequence and the volume change of protein folding.
Size and sequence and the volume change of protein folding.
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DOI：
10.1021/ja200228w
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发表时间：
2011-04-20
影响因子：
15
通讯作者：
Royer, Catherine A.
中科院分区：
文献类型：
--
作者：
Rouget, Jean-Baptiste;Aksel, Tural;Roche, Julien;Saldana, Jean-Louis;Garcia, Angel E.;Barrick, Doug;Royer, Catherine A.
根据LeChatelier原理，静水压力的应用通常会导致蛋白质展开，这意味着展开状态的摩尔体积比折叠状态的摩尔体积小。然而，展开时体积变化的来源ΔVu尚未确定。我们系统地研究了蛋白质大小和序列对ΔVu值的影响，并以NotchR的一系列缺失突变体为模型系统。这一结果有力地支持了蛋白质压力效应的主要因素是折叠状态下不完善的内部堆积的观点。这些填充缺陷似乎特定地定位在3D结构中，与温度和变性剂的均匀分布效应形成对比，后者依赖于展开时暴露表面积的水合作用。鉴于其局部性质，全球压力影响蛋白质结构的程度可以决定折叠状态固有的协作性和长程耦合程度。我们还表明，蛋白质构象的能量学可以显著调节它们的体积性质，为进一步了解蛋白质的稳定性提供了进一步的见解。
The application of hydrostatic pressure generally leads to protein unfolding, implying, in accordance to LeChatelier’s principle, that the unfolded state has smaller molar volume than the folded state. However, the origin of the volume change upon unfolding, ΔVu, has yet to be determined. We have examined systematically the effects of protein size and sequence on the value of ΔVu using as a model system a series of deletion variants of the ankyrin repeat domain of the Notch receptor. The results provide strong evidence in support of the notion that the major contributing factor to pressure effects on proteins is their imperfect internal packing in the folded state. These packing defects appear to be specifically localized in the 3D structure, in contrast to the uniformly distributed effects of temperature and denaturants which depend upon hydration of exposed surface area upon unfolding. Given its local nature, the extent to which pressure globally affects protein structure can inform on the degree of cooperativity and long range coupling intrinsic to the folded state. We also show that the energetics of the protein’s conformations can significantly modulate their volumetric properties, providing further insight into protein stability.
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影响因子：
3
作者：
Ahmad S;Gromiha M;Fawareh H;Sarai A
通讯作者：
Sarai A
影响因子：
5.6
作者：
Bradley, CM;Barrick, D
通讯作者：
Barrick, D
影响因子：
2.9
作者：
HOOVER, WG
通讯作者：
HOOVER, WG
DOI：
10.1046/j.1432-1327.1999.00751.x
发表时间：
1999-10-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Mei, G;Di Venere, A;Finazzi-Agrò, A
通讯作者：
Finazzi-Agrò, A
影响因子：
2.9
作者：
HAWLEY, SA
通讯作者：
HAWLEY, SA