Identification of a pluripotency-inducing small compound, PLU, that induces callus formation via Heat Shock Protein 90-mediated activation of auxin signaling.
Identification of a pluripotency-inducing small compound, PLU, that induces callus formation via Heat Shock Protein 90-mediated activation of auxin signaling.
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DOI：
10.3389/fpls.2023.1099587
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发表时间：
2023
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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植物通过体细胞的去分化，保留了产生多能组织的能力，即愈伤组织。用生长素和细胞分裂素的激素混合物培养外植体也可以人工诱导多能愈伤组织，然后从愈伤组织中再生出一个完整的身体。在这里，我们发现了一种多能诱导的小化合物，PLU，在不外用生长素或细胞分裂素的情况下，诱导形成具有组织再生能力的愈伤组织。plus诱导的愈伤组织通过侧根形成过程表达了与多能性获得相关的几个标记基因。PLU诱导的愈伤组织形成需要激活生长素信号通路，尽管PLU处理降低了活性生长素的数量。RNA-seq分析和随后的实验表明，热休克蛋白90 （HSP90）介导了plus启动的早期事件的重要部分。我们还发现，hsp90依赖性诱导的运输抑制剂应答1（一种生长素受体基因）是PLU形成愈伤组织所必需的。综上所述，本研究为从不同的角度操纵和研究植物多能性诱导提供了一种新的工具，而不是传统的外用激素混合物的方法。
Plants retain the ability to generate a pluripotent tissue called callus by dedifferentiating somatic cells. A pluripotent callus can also be artificially induced by culturing explants with hormone mixtures of auxin and cytokinin, and an entire body can then be regenerated from the callus. Here we identified a pluripotency-inducing small compound, PLU, that induces the formation of callus with tissue regeneration potency without the external application of either auxin or cytokinin. The PLU-induced callus expressed several marker genes related to pluripotency acquisition via lateral root initiation processes. PLU-induced callus formation required activation of the auxin signaling pathway though the amount of active auxin was reduced by PLU treatment. RNA-seq analysis and subsequent experiments revealed that Heat Shock Protein 90 (HSP90) mediates a significant part of the PLU-initiated early events. We also showed that HSP90-dependent induction of TRANSPORT INHIBITOR RESPONSE 1, an auxin receptor gene, is required for the callus formation by PLU. Collectively, this study provides a new tool for manipulating and investigating the induction of plant pluripotency from a different angle from the conventional method with the external application of hormone mixtures.
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影响因子：
3.7
作者：
Windels D;Bielewicz D;Ebneter M;Jarmolowski A;Szweykowska-Kulinska Z;Vazquez F
通讯作者：
Vazquez F
影响因子：
4.9
作者：
Chen, Zhehao;Hu, Lingzhi;Wang, Lilin
通讯作者：
Wang, Lilin
影响因子：
5.6
作者：
Garrido-Vargas F;Godoy T;Tejos R;O'Brien JA
通讯作者：
O'Brien JA
影响因子：
64.5
作者：
DiLaurenzio, L;WysockaDiller, J;Benfey, PN
通讯作者：
Benfey, PN
影响因子：
4.6
作者：
He Q;Yang L;Hu W;Zhang J;Xing Y
通讯作者：
Xing Y