The X-linked imprinted gene family Fthl17 shows predominantly female expression following the two-cell stage in mouse embryos.
The X-linked imprinted gene family Fthl17 shows predominantly female expression following the two-cell stage in mouse embryos.
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DOI：
10.1093/nar/gkq113
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发表时间：
2010-06
影响因子：
14.9
通讯作者：
Okabe M
中科院分区：
文献类型：
--
作者：
Kobayashi S;Fujihara Y;Mise N;Kaseda K;Abe K;Ishino F;Okabe M
雄性和雌性哺乳动物之间的差异是由于胚胎将性腺分化为睾丸或卵巢。然而，这可能不是唯一的决定因素。有报道称，胚胎性分化可能先于性腺分化并独立于性腺分化，但很少有分子生物学证据支持这一点。为了测试早期胚胎的性别差异，我们使用新开发的非侵入性性别鉴定方法对表达绿色荧光蛋白的转基因小鼠进行了雄性和雌性囊胚的分离，并比较了基因表达模式。通过这次筛选，我们发现Fthl17(铁蛋白，重多肽样17)家族基因主要在雌性囊胚中表达。这包括聚集在X染色体上的七个基因。基于DNA多态性的表达分析表明，这些基因早在双细胞期就在父本X染色体上印记和表达。因此，当合子基因组激活开始时，雄性和雌性小鼠胚胎之间的基因表达已经存在差异。这一发现对早期性别分化的研究将是重要的，因为这些差异显然是在性腺分化之前出现的。
Differences between male and female mammals are initiated by embryonic differentiation of the gonad into either a testis or an ovary. However, this may not be the sole determinant. There are reports that embryonic sex differentiation might precede and be independent of gonadal differentiation, but there is little molecular biological evidence for this. To test for sex differences in early-stage embryos, we separated male and female blastocysts using newly developed non-invasive sexing methods for transgenic mice expressing green fluorescent protein and compared the gene-expression patterns. From this screening, we found that the Fthl17 (ferritin, heavy polypeptide-like 17) family of genes was predominantly expressed in female blastocysts. This comprises seven genes that cluster on the X chromosome. Expression analysis based on DNA polymorphisms revealed that these genes are imprinted and expressed from the paternal X chromosome as early as the two-cell stage. Thus, by the time zygotic genome activation starts there are already differences in gene expression between male and female mouse embryos. This discovery will be important for the study of early sex differentiation, as clearly these differences arise before gonadal differentiation.
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影响因子：
3.6
作者：
BEYER, C;EUSTERSCHULTE, B;REISERT, I
通讯作者：
REISERT, I
影响因子：
4.4
作者：
Nakanishi, T;Kuroiwa, A;Okabe, M
通讯作者：
Okabe, M
影响因子：
64.5
作者：
MacLean, JA;Chen, MGA;Wilkinson, MF
通讯作者：
Wilkinson, MF
影响因子：
4.4
作者：
McDonald, LE;Paterson, CA;Kay, GF
通讯作者：
Kay, GF
DOI：
10.1002/ajmg.10022
发表时间：
2001-12-01
期刊：
AMERICAN JOURNAL OF MEDICAL GENETICS
影响因子：
--
作者：
Kobayashi, S;Uemura, H;Ishino, F
通讯作者：
Ishino, F