Synergistic effects of fibroblast growth factor-2 and bone morphogenetic protein-2 on bone induction.
Synergistic effects of fibroblast growth factor-2 and bone morphogenetic protein-2 on bone induction.
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成纤维细胞生长因子-2和骨形态发生蛋白-2对骨诱导的协同作用
DOI：
10.3892/mmr.2017.7183
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发表时间：
2017-10
影响因子：
3.4
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Song R;Wang D;Zeng R;Wang J
本研究调查了共同施用成纤维细胞生长因子-2 (FGF-2) 和骨形态发生蛋白-2 (BMP-2) 对 C2C12 细胞和大鼠成骨细胞分化的协同作用。 C2C12 小鼠成肌细胞代表了一种广为接受的体外模型系统，用于研究 BMP-2 将细胞谱系从生肌表型改变为成骨表型的能力。通过碱性磷酸酶（ALP）和茜素红S染色测定成骨细胞分化能力。比色法测量 ALP 活性和钙浓度。同时给予 4 µg/ml 重组人 BMP-2 和 2 ng/ml FGF-2 可显着增强 C2C12 细胞的 ALP 活性（成骨的早期标志物）。这种组合还增加了细胞外信号调节激酶1/2丝裂原激活蛋白激酶信号传导，该信号传导参与 FGF-2 对 BMP-2 诱导的 C2C12 细胞成骨细胞分化的促进作用。在大鼠异位植入模型中，通过使用胶原蛋白和壳聚糖包被的抗原提取猪松质植入物同时局部持续施用 FGF-2 和 BMP-2，可以最佳地促进钙沉积（成骨的晚期标志物）和 CD34（新血管的标志物）的表达。 BMP-2 和 FGF-2 组合的协同作用可能具有骨再生治疗的潜力。
The present study investigated the synergistic effect of co-administering fibroblast growth factor-2 (FGF-2) and bone morphogenetic protein-2 (BMP-2) on osteoblastic differentiation in C2C12 cells and in rats. C2C12 murine myoblast cells represent a well-accepted in vitro model system to study the ability of BMP-2 to alter cell lineage from the myogenic to the osteogenic phenotype. The osteoblastic differentiation potency was determined by alkaline phosphatase (ALP) and Alizarin red S staining. ALP activity and calcium concentrations were colorimetrically measured. Simultaneous administration of 4 µg/ml recombinant human BMP-2 with 2 ng/ml FGF-2 markedly enhanced ALP activity (an early marker of osteogenesis) of C2C12 cells. This combination also increased extracellular signal-regulated kinase1/2 mitogen activated protein kinase signaling that is involved in the promoting effect of FGF-2 on BMP-2-induced osteoblastic differentiation in C2C12 cells. Calcium deposition (a late marker of osteogenesis) and the expression of CD34 (a marker of new vessels) were promoted optimally by simultaneous local sustained administration of FGF-2 and BMP-2 using collagen and chitosan-coated antigen-extracted porcine cancellous implants in a rat ectopic implantation model. The synergistic effects of a combination of BMP-2 and FGF-2 may have potential for bone regenerative therapeutics.
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影响因子：
3.1
作者：
Ma, Shu-Yuan;Feng, Zhi-Qiang;Yin, Zhong-Da
通讯作者：
Yin, Zhong-Da
影响因子：
9.7
作者：
Perez, R. A.;El-Fiqi, A.;Kim, H. -W.
通讯作者：
Kim, H. -W.
影响因子：
2.8
作者：
Kawaguchi, Hiroshi;Jingushi, Seiya;Nakamura, Kozo
通讯作者：
Nakamura, Kozo
影响因子：
4.9
作者：
Atluri K;Seabold D;Hong L;Elangovan S;Salem AK
通讯作者：
Salem AK
影响因子：
9.7
作者：
Yuan, Q.;Kubo, T.;Akagawa, Y.
通讯作者：
Akagawa, Y.