Functional Restoration of Bacteriomes and Viromes by Fecal Microbiota Transplantation.
Functional Restoration of Bacteriomes and Viromes by Fecal Microbiota Transplantation.
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DOI：
10.1053/j.gastro.2021.02.013
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发表时间：
2021-05
期刊：
影响因子：
29.4
通讯作者：
Uematsu S
中科院分区：
文献类型：
--
作者：
Fujimoto K;Kimura Y;Allegretti JR;Yamamoto M;Zhang YZ;Katayama K;Tremmel G;Kawaguchi Y;Shimohigoshi M;Hayashi T;Uematsu M;Yamaguchi K;Furukawa Y;Akiyama Y;Yamaguchi R;Crowe SE;Ernst PB;Miyano S;Kiyono H;Imoto S;Uematsu S
粪便微生物群移植（FMT）是治疗复发性艰难梭菌感染（rCDI）的有效方法。然而，FMT成功的整体机制有待全面阐明，FMT的安全性最近已成为一个严重的问题，因为耐药菌血症的发生FMT传播。我们研究了FMT是否能恢复细菌组和病毒组的功能，这可能是FMT成功的指标。分析了9例成功接受FMT的rCDI患者及其供体的人肠道细菌组和病毒组。检查了FMT之前和之后来自接受者的样品中以及供体样品中基于原噬菌体和基于CRISPR间隔子的宿主细菌-噬菌体缔合。评价了FMT对肠道微生物基因功能的影响。病毒组和细菌组的宏基因组测序显示，与FMT前相比，FMT确实改变了FMT后受体肠道细菌组和病毒组的特征。特别是，许多变形菌，粪便丰度高FMT前，被淘汰，和微病毒科的比例增加受体。大多数温带真菌也表现出与被FMT改变的宿主细菌平行的行为。此外，细菌和病毒基因功能的鉴定前后FMT显示，一些独特的途径，包括氟苯甲酸降解和二级胆汁酸生物合成，显着代表。肠道菌群与宿主菌的协同作用恢复了受体肠道植物群。这些发现表明，肠道菌群功能的恢复反映了FMT的成功。
Fecal microbiota transplantation (FMT) is an effective therapy for recurrent Clostridioides difficile infection (rCDI). However, the overall mechanisms underlying FMT success await comprehensive elucidation, and the safety of FMT has recently become a serious concern because of the occurrence of drug-resistant bacteremia transmitted by FMT. We investigated whether functional restoration of the bacteriomes and viromes by FMT could be an indicator of successful FMT. The human intestinal bacteriomes and viromes from 9 patients with rCDI who had undergone successful FMT and their donors were analyzed. Prophage-based and CRISPR spacer-based host bacteria-phage associations in samples from recipients before and after FMT and in donor samples were examined. The gene functions of intestinal microorganisms affected by FMT were evaluated. Metagenomic sequencing of both the viromes and bacteriomes revealed that FMT does change the characteristics of intestinal bacteriomes and viromes in recipients after FMT compared with those before FMT. In particular, many Proteobacteria, the fecal abundance of which was high before FMT, were eliminated, and the proportion of Microviridae increased in recipients. Most temperate phages also behaved in parallel with the host bacteria that were altered by FMT. Furthermore, the identification of bacterial and viral gene functions before and after FMT revealed that some distinctive pathways, including fluorobenzoate degradation and secondary bile acid biosynthesis, were significantly represented. The coordinated action of phages and their host bacteria restored the recipients’ intestinal flora. These findings show that the restoration of intestinal microflora functions reflects the success of FMT.
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影响因子：
2.7
作者：
Gregor I;Dröge J;Schirmer M;Quince C;McHardy AC
通讯作者：
McHardy AC
微生物群转移疗法改变肠道生态系统并改善胃肠道和自闭症症状：一项开放标签研究。
影响因子：
15.5
作者：
Kang DW;Adams JB;Gregory AC;Borody T;Chittick L;Fasano A;Khoruts A;Geis E;Maldonado J;McDonough-Means S;Pollard EL;Roux S;Sadowsky MJ;Lipson KS;Sullivan MB;Caporaso JG;Krajmalnik-Brown R
通讯作者：
Krajmalnik-Brown R
影响因子：
56.9
作者：
Li, Simone S.;Zhu, Ana;Bork, Peer
通讯作者：
Bork, Peer
影响因子：
11.8
作者：
McDonald, L. Clifford;Gerding, Dale N.;Wilcox, Mark H.
通讯作者：
Wilcox, Mark H.
影响因子：
3.7
作者：
Moss, Eli L.;Falconer, Shannon B.;Bhatt, Ami S.
通讯作者：
Bhatt, Ami S.