The kinetics of sulfobromophthalein uptake by rat liver sinusoidal vesicles.
The kinetics of sulfobromophthalein uptake by rat liver sinusoidal vesicles.
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大鼠肝窦小泡摄取磺溴酞的动力学。
DOI：
10.1016/0005-2736(87)90034-4
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Berk,PD
中科院分区：
文献类型：
--
作者：
Potter,BJ;Blades,BF;Shepard,MD;Thung,SM;Berk,PD
利用以K+为主要内部无机阳离子的分离大鼠肝窦膜囊泡，研究了溴[35 S]磺胺吡啶（35 S- bsp）在肝细胞窦膜上的结合和摄取动力学。35 S-BSP进入囊泡的吸收量与温度有关，最大吸收量在35至40°C之间；在37°C时，通过Eadee-Hofstee分析可将其饱和分解为两个组分：高亲和力（K m= 53.1 μ m）但容量低，低亲和力（K m= 1150 μ m）但容量高。在未标记的BSP孵卵前或孵卵后，35 S-BSP的反式刺激和反式转运也可以在这些囊泡中得到证实。5 μM的玫瑰红和10 μM的吲哚菁绿抑制了竞争性摄取，10 μM的DIDS抑制了非竞争性摄取。当浓度≥250 μ m时，牛磺胆酸盐不抑制摄取，实际上增强了转运。当氯离子是这个梯度的一部分时，施加向内的无机离子梯度导致35 S-BSP转运增强，而不管所采用的是哪种阳离子，而没有明显的阳离子效应。然而，用10mm Na+代替10mm K+作为内部阳离子，与Na+梯度相比，在外部K+存在的情况下，吸收显著增加。当使用10 mM Tris+作为内部阳离子时，没有观察到这种效应。35 S-BSP被孤立的正弦膜囊吸收的动力学表明运输方便。虽然观察到的无机离子效应表明可能存在致电成分，但肝脏摄取BSP的驱动力仍不确定。分离的窦状膜囊泡为研究独立于循环或随后的细胞现象的肝脏摄取过程提供了有用的技术。
The kinetics of bromo [35 S] sulfophthalein (35 S-BSP) binding by and uptake across the hepatocyte sinusoidal membrane were investigated using isolated rat liver sinusoidal membrane vesicles containing K+ as the principal internal inorganic cation. Uptake of 35 S-BSP into vesicles was found to be temperature dependent, with maximum uptake between 35 and 40° C; only binding occurred at or below 15° C. Uptake at 37° C was saturable and resolvable by Eadee-Hofstee analysis into two components: one with high affinity (K m= 53.1 μ M) but low capacity, and the second of low affinity (K m= 1150 μ M) but high capacity. By pre-or post-incubation, respectively, with unlabelled BSP, trans-stimulation and counter transport of 35 S-BSP could also be demonstrated in these vesicles. Uptake was inhibited competitively using 5 μM Rose bengal and 10 μM indocyanine green, and non-competitively using 10 μM DIDS. Taurocholate did not inhibit uptake, and actually enhanced transport at concentrations≥ 250 μ M. Imposition of inwardly directed inorganic ion gradients resulted in the enhancement of 35 S-BSP transport when chloride ions were part of this gradient, irrespective of the cation employed whereas there was no apparent cation effect. However, substitution of 10 mM Na+ for 10 mM K+ as the internal cation resulted in a significant increase in uptake in the presence of external K+ as compared to Na+ gradients. This effect was not observed when 10 mM Tris+ was employed as the internal cation. The kinetics of 35 S-BSP uptake by isolated sinusoidal membrane vesicles are indicative of facilitated transport. While the observed inorganic ion effects suggest a possible electrogenic component, the driving forces for hepatic BSP uptake remain uncertain. Isolated sinusoidal membrane vesicles provide a useful technique for studying hepatic uptake processes independent of circulatory or subsequent cellular phenomena.
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白蛋白结合不促进分离的灌注大鼠肝脏中肝胆红素的摄取。
DOI：
10.1172/jci111021
发表时间：
1983
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Stollman,YR;Gartner,U;Theilmann,L;Ohmi,N;Wolkoff,AW
通讯作者：
Wolkoff,AW
验证用于测量膜相关 Mg-和 NaK-ATP 酶活性的记录分光光度法。
DOI：
--
发表时间：
1979
期刊：
The Journal of laboratory and clinical medicine
影响因子：
--
作者：
B. Scharschmidt;Keeffe Eb;N. Blankenship;Ockner Rk
通讯作者：
Ockner Rk
DOI：
10.1016/0304-4165(83)90019-3
发表时间：
1983-03
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
W. Stremmel;B. Potter;P. Berk
通讯作者：
W. Stremmel;B. Potter;P. Berk
影响因子：
56.9
作者：
WEISIGER, R;GOLLAN, J;OCKNER, R
通讯作者：
OCKNER, R
将有机阴离子转运至肝细胞和胆汁中。
影响因子：
5.8
作者：
D. Rollins;J. Freston;D. M. Woodbury
通讯作者：
D. M. Woodbury