Identification of essential alphaproteobacterial genes reveals operational variability in conserved developmental and cell cycle systems.
Identification of essential alphaproteobacterial genes reveals operational variability in conserved developmental and cell cycle systems.
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DOI：
10.1111/mmi.12686
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发表时间：
2014-08
影响因子：
3.6
通讯作者：
Brun YV
中科院分区：
文献类型：
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作者：
Curtis PD;Brun YV
新月柄杆菌的细胞周期由协调事件的复杂信号网络控制。基因组测序显示，许多新月弯孢菌细胞周期基因在其他阿尔法变形菌中是保守的，但尚不清楚它们的功能保守到什么程度。由于许多细胞周期调控基因在 C. crescentus 中是必需的，因此阐明了两种 Alphaproteobacteria、根瘤农杆菌 (Rhizobiales) 和亚弧菌短波单胞菌 (Caulobacterales) 的必需基因，以识别不同系统发育距离内细胞周期蛋白功能的变化，如重要性变化所证明的那样。结果表明，大多数保守的必需基因参与关键的细胞周期过程。成分重要性的变化反映了生活方式的重大变化，例如根癌农杆菌中由于该生物体的不同生长模式而产生的分裂成分。在细胞周期调节因子中观察到了更大的重要性变异性，这表明调节机制比它们调节的过程更容易定制。例子包括 divJ 和 divK 空间细胞周期调节因子的重要性的可变性，以及高度保守且通常是必需的 DNA 甲基转移酶 CcrM 的非必需性。这些结果表明，虽然基本细胞功能在不同的遗传距离上是保守的，但给定生物体的大部分基本基因库是该生物体特有的。
The cell cycle of Caulobacter crescentus is controlled by a complex signaling network that coordinates events. Genome sequencing has revealed many C. crescentus cell cycle genes are conserved in other Alphaproteobacteria, but it is not clear to what extent their function is conserved. As many cell cycle regulatory genes are essential in C. crescentus, the essential genes of two Alphaproteobacteria, Agrobacterium tumefaciens (Rhizobiales) and Brevundimonas subvibrioides (Caulobacterales), were elucidated to identify changes in cell cycle protein function over different phylogenetic distances as demonstrated by changes in essentiality. The results show the majority of conserved essential genes are involved in critical cell cycle processes. Changes in component essentiality reflect major changes in lifestyle, such as divisome components in A. tumefaciens resulting from that organism’s different growth pattern. Larger variability of essentiality was observed in cell cycle regulators, suggesting regulatory mechanisms are more customizable than the processes they regulate. Examples include variability in the essentiality of divJ and divK spatial cell cycle regulators, and non-essentiality of the highly conserved and usually essential DNA methyltransferase CcrM. These results show that while essential cell functions are conserved across varying genetic distance, much of a given organism’s essential gene pool is specific to that organism.
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影响因子：
4.5
作者：
Fioravanti A;Fumeaux C;Mohapatra SS;Bompard C;Brilli M;Frandi A;Castric V;Villeret V;Viollier PH;Biondi EG
通讯作者：
Biondi EG
影响因子：
9.9
作者：
通讯作者：
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影响因子：
3.6
作者：
Curtis PD;Quardokus EM;Lawler ML;Guo X;Klein D;Chen JC;Arnold RJ;Brun YV
通讯作者：
Brun YV
影响因子：
4.2
作者：
Curtis, Patrick D.;Klein, David;Brun, Yves V.
通讯作者：
Brun, Yves V.
大肠杆菌K-12的构造框架，单基因敲除突变体：Keio Collection。
影响因子：
9.9
作者：
通讯作者：
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