The scaffolding and signalling functions of a localization factor impact polar development.
The scaffolding and signalling functions of a localization factor impact polar development.
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DOI：
10.1111/j.1365-2958.2012.08055.x
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发表时间：
2012-05
影响因子：
3.6
通讯作者：
Brun YV
中科院分区：
文献类型：
--
作者：
Curtis PD;Quardokus EM;Lawler ML;Guo X;Klein D;Chen JC;Arnold RJ;Brun YV
在分化中的α-变形杆菌Caulobacter crescentus中，细胞极的细胞器合成对于细胞分裂后形成不同的后代至关重要。极性细胞器合成的协调，包括皮利和固着，和鞭毛弹射，部分介导的支架蛋白PodJ。在细胞分裂的时候，PodJ经过调节加工成一个短的形式，持续存在于蜂群细胞的鞭毛极。本研究分析了PodJ在结构和信号蛋白定位中的作用如何影响细胞器合成。具有内部缺失的PodJ突变体表现出对嗜毛性噬菌体ΦCbK的敏感性降低，这是由于pilA基因表达降低，这可能与改变的信号蛋白定位有关。ΔpodJ突变体的噬菌体敏感性缺陷可以通过异位pilA表达部分抑制。通过操纵podJ本身或控制perP表达诱导PodJ加工，导致菌毛生物发生减少，并且当与减少pilA表达的podJ突变结合时，导致噬菌体敏感性完全丧失。总体而言，结果表明，PodJ的支架作用的结构和信号蛋白都有助于鞭毛极细胞器的发展。
In the differentiating alphaproteobacterium Caulobacter crescentus, organelle synthesis at cell poles is critical to forming different progeny after cell division. Coordination of polar organelle synthesis, including pili and holdfast, and flagellum ejection, is mediated in part by the scaffolding protein PodJ. At the time of cell division, PodJ undergoes regulated processing to a short form that persists at the flagellar pole of swarmer cells. This study analyzes how PodJ’s role in structural and signaling protein localization impacts organelle synthesis. A PodJ mutant with an internal deletion exhibits reduced sensitivity to pili-tropic phage ΦCbK, resulting from reduced pilA gene expression, which can be linked to altered signaling protein localization. The phage sensitivity defect of a ΔpodJ mutant can be partially suppressed by ectopic pilA expression. Induction of PodJ processing, by manipulation of podJ itself or controlled perP expression, resulted in decreased pilus biogenesis and, when coupled with a podJ mutation that reduced pilA expression, led to complete loss of phage sensitivity. As a whole, the results show that PodJ’s scaffolding role for structural and signaling proteins both contribute to flagellar pole organelle development.
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影响因子：
3.6
作者：
Alba, BM;Zhong, HJ;Gross, CA
通讯作者：
Gross, CA
DOI：
10.1073/pnas.051609998
发表时间：
2001-03-27
影响因子：
11.1
作者：
Jacobs, C;Hung, D;Shapiro, L
通讯作者：
Shapiro, L
影响因子：
16
作者：
Flynn, JM;Neher, SB;Baker, TA
通讯作者：
Baker, TA
影响因子：
3.2
作者：
Bodenmiller, D;Toh, E;Brun, YV
通讯作者：
Brun, YV
影响因子：
3.6
作者：
Bellefontaine, AF;Pierreux, CE;De Bolle, X
通讯作者：
De Bolle, X