Understanding behavioral and physiological phenotypes of stress and anxiety in zebrafish.
Understanding behavioral and physiological phenotypes of stress and anxiety in zebrafish.
复制标题
DOI：
10.1016/j.bbr.2009.06.022
复制
发表时间：
2009-12-14
影响因子：
2.7
通讯作者：
Kalueff, Allan V.
中科院分区：
文献类型：
--
作者：
Egan, Rupert J.;Bergner, Carisa L.;Hart, Peter C.;Cachat, Jonathan M.;Canavello, Peter R.;Elegante, Marco F.;Elkhayat, Salem I.;Bartels, Brett K.;Tien, Anna K.;Tien, David H.;Mohnot, Sopan;Beeson, Esther;Glasgow, Eric;Amri, Hakima;Zukowska, Zofia;Kalueff, Allan V.
斑马鱼（Danio rerio）正在成为压力和焦虑实验研究的一种有前途的模式生物。在这里，我们进一步验证斑马鱼模型的压力，分析环境和药理学操作如何影响他们的行为和生理表型。实验操作包括暴露于报警信息素，慢性暴露于氟西汀，急性暴露于咖啡因，以及急性和慢性暴露于乙醇。急性（但不是慢性）警报信息素和急性咖啡因产生强大的焦虑作用，包括减少探索，增加不稳定的运动和冻结行为的斑马鱼在新的坦克潜水测试。相比之下，乙醇和氟西汀具有强大的抗焦虑作用，包括增加探索和减少不稳定的运动。还对几种斑马鱼品系的行为进行了定量，以确定它们的行为特征的差异，揭示了高焦虑（豹，白化病）和低焦虑（野生型）品系。我们还使用了LocoScan（CleverSys Inc.）视频跟踪工具来量化斑马鱼的焦虑相关行为，并从自发活动中剖析焦虑相关的表型。最后，我们开发了一种简单有效的方法来测量斑马鱼的生理应激反应（基于人类唾液皮质醇测定），并表明斑马鱼全身皮质醇水平的变化与焦虑的行为指数平行。总的来说，我们的研究结果证实了斑马鱼作为一个有效的，可靠的，高通量的压力和情感障碍模型。
The zebrafish (Danio rerio) is emerging as a promising model organism for experimental studies of stress and anxiety. Here we further validate zebrafish models of stress by analyzing how environmental and pharmacological manipulations affect their behavioral and physiological phenotypes. Experimental manipulations included exposure to alarm pheromone, chronic exposure to fluoxetine, acute exposure to caffeine, as well as acute and chronic exposure to ethanol. Acute (but not chronic) alarm pheromone and acute caffeine produced robust anxiogenic effects, including reduced exploration, increased erratic movements and freezing behavior in zebrafish tested in the novel tank diving test. In contrast, ethanol and fluoxetine had robust anxiolytic effects, including increased exploration and reduced erratic movements. The behavior of several zebrafish strains was also quantified to ascertain differences in their behavioral profiles, revealing high-anxiety (leopard, albino) and low-anxiety (wild type) strains. We also used LocoScan (CleverSys Inc.) video-tracking tool to quantify anxiety-related behaviors in zebrafish, and dissect anxiety-related phenotypes from locomotor activity. Finally, we developed a simple and effective method of measuring zebrafish physiological stress responses (based on a human salivary cortisol assay), and showed that alterations in whole-body cortisol levels in zebrafish parallel behavioral indices of anxiety. Collectively, our results confirm zebrafish as a valid, reliable, and high-throughput model of stress and affective disorders.
登录
查看更多内容
影响因子：
1.3
作者：
Colwill, RM;Raymond, MP;Escudero, H
通讯作者：
Escudero, H
影响因子：
4
作者：
Dooley, K;Zon, LI
通讯作者：
Zon, LI
DOI：
10.1073/pnas.191380698
发表时间：
2001-09-25
影响因子：
11.1
作者：
Darland, T;Dowling, JE
通讯作者：
Dowling, JE
影响因子：
2.9
作者：
Airhart, Mark J.;Lee, Deborah H.;Skalko, Richard G.
通讯作者：
Skalko, Richard G.
影响因子：
2.5
作者：
Houchi, H.;Warnault, V.;Naassila, M.
通讯作者：
Naassila, M.