Up-regulation and pre-activation of TRAF3 and TRAF5 in inflammatory bowel disease.
Up-regulation and pre-activation of TRAF3 and TRAF5 in inflammatory bowel disease.
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炎症性肠病中 TRAF3 和 TRAF5 的上调和预激活
DOI：
10.7150/ijms.5457
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发表时间：
2013
影响因子：
3.6
通讯作者：
Wang TR
中科院分区：
文献类型：
--
作者：
Shen J;Qiao YQ;Ran ZH;Wang TR
目的：TRAF3和TRAF5具有共同的祖先基因，作为免疫信号通路的重要组成部分相互作用。TRAF3和TRAF5在结肠炎大鼠/小鼠模型的结肠中过表达。然而，TRAF3和TRAF5在炎症性肠病患者中的表达尚未阐明。本研究的目的是探讨TRAF3和TRAF5在炎症性肠病患者中的潜在作用。方法：采用酶联免疫吸附法（ELISA）检测血浆TRAF3和TRAF5蛋白水平。Western blot检测TRAF3和TRAF5蛋白在结肠中的表达。应用定量实时PCR（qRT-PCR）进行基因表达。对炎症性肠病患者的炎症肠粘膜和非炎症肠粘膜以及健康对照的正常粘膜进行分析。结果如下：克罗恩病和溃疡性结肠炎患者的TRAF3和TRAF5的血浆水平均显著高于健康对照组。在溃疡性结肠炎患者中，只有可溶性TRAF5与内镜疾病活动指数（Baron评分）显示弱相关性（Spearman's r = 0.358，P = 0.022）。克罗恩病和溃疡性结肠炎患者外周血单个核细胞TRAF3和TRAF5基因表达均显著高于健康对照组（P均<0.0001）。TRAF3和TRAF5基因和蛋白在克罗恩病和溃疡性结肠炎患者的炎症结肠粘膜中的表达显著高于非炎症结肠粘膜和健康对照者的正常结肠粘膜（均P <0.0001）。此外，TRAF3和TRAF5的基因和蛋白表达在克罗恩病和溃疡性结肠炎患者的非炎症结肠粘膜中也显著高于健康对照的正常粘膜。结论：TRAF3和TRAF5在炎症性肠病中过表达。虽然内窥镜检查的外观可以是正常的，但TRAF3和TRAF5预激活可以在非炎症结肠段中检测到。
Objective: TRAF3 and TRAF5 share a common ancestral gene, and interact as essential components of signaling pathways in immunity. TRAF3 and TRAF5 are overexpressed in the colon of rat/mouse models with colitis. However, the expressions of TRAF3 and TRAF5 in patients with inflammatory bowel disease have not been elucidated. The aim of the present study is to explore the potential roles of TRAF3 and TRAF5 in patients with inflammatory bowel disease. Methods: Plasma levels of TRAF3 and TRAF5 proteins were detected by Enzyme-linked Immunosorbent Assay (ELISA). Colonic expression of TRAF3 and TRAF5 proteins was detected by western blot analysis. Quantitative Real-time PCR (qRT-PCR) was applied for gene expression. Inflamed intestinal mucosa and non-inflamed intestinal mucosa in patients with inflammatory bowel disease and normal mucosa was analyzed from healthy controls. Results: The plasma levels of TRAF3 and TRAF5 were significantly higher both in patients with Crohn's disease and ulcerative colitis than in healthy controls. Only soluble TRAF5 showed a weak correlation with endoscopic disease activity index (Baron score) in patients with ulcerative colitis (spearman's r=0.358, P=0.022). Gene expressions of TRAF3 and TRAF5 in peripheral blood mononuclear cells were significantly higher both in patients with Crohn's disease and ulcerative colitis than in healthy controls (all P<0.0001). Gene and protein expressions of TRAF3 and TRAF5 were significantly higher in inflamed colonic mucosa of patients with Crohn's disease and ulcerative colitis than in non-inflamed colonic mucosa and normal mucosa of healthy controls (all P<0.0001). Furthermore, gene and protein expressions of TRAF3 and TRAF5 were also significantly higher in non-inflamed colonic mucosa of patients with Crohn's disease and ulcerative colitis than in normal mucosa of healthy controls. Conclusions: TRAF3 and TRAF5 are overexpressed in inflammatory bowel disease. Although the endoscopic appearance can be normal, TRAF3 and TRAF5 pre-activation can be detected in non-inflamed colonic segments.
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影响因子：
30.5
作者：
通讯作者：
--
DOI：
10.2174/187221310791163071
发表时间：
2010-01-01
影响因子：
4.2
作者：
Wei, Jia;Feng, Jiexiong
通讯作者：
Feng, Jiexiong
影响因子：
7.7
作者：
Elmunzer, B. Joseph;Higgins, Peter D. R.;Elta, Grace H.
通讯作者：
Elta, Grace H.
影响因子：
4.8
作者：
Wixted, Josephine H. F.;Rothstein, Jay L.;Eisenlohr, Laurence C.
通讯作者：
Eisenlohr, Laurence C.
影响因子：
32.4
作者：
Pérez de Diego R;Sancho-Shimizu V;Lorenzo L;Puel A;Plancoulaine S;Picard C;Herman M;Cardon A;Durandy A;Bustamante J;Vallabhapurapu S;Bravo J;Warnatz K;Chaix Y;Cascarrigny F;Lebon P;Rozenberg F;Karin M;Tardieu M;Al-Muhsen S;Jouanguy E;Zhang SY;Abel L;Casanova JL
通讯作者：
Casanova JL