Comparative analyses of the soft tissue interfaces around teeth and implants: Insights from a pre-clinical implant model.
Comparative analyses of the soft tissue interfaces around teeth and implants: Insights from a pre-clinical implant model.
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DOI：
10.1111/jcpe.13446
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发表时间：
2021-05
影响因子：
6.7
通讯作者：
Helms JA
中科院分区：
文献类型：
--
作者：
Yuan X;Pei X;Chen J;Zhao Y;Brunski JB;Helms JA
评价种植体周围上皮（PIE）与天然结合上皮（JE）屏障功能的相似性和差异。使用小鼠模型，其中钛种植体被放置在愈合的拔牙部位的咬合下。在种植体植入后的多个时间点检查PIE，以捕获和了解其组装和稳态的时间性质。有丝分裂活性，半桥粒附着装置，并在PIE炎症反应进行了比较对JE。此外，我们评估了PIE是否像JE一样发展了Wnt反应性干细胞小生境。PIE由口腔上皮（OE）发展而来，在植入种植体时，口腔上皮（OE）失去了JE的所有特征。与JE相比，建立的PIE具有更多的增殖细胞，表现出较低的附着蛋白表达，并且在下层结缔组织中具有显著更多的炎性细胞。OE中的Wnt响应细胞有助于最初的PIE，但随着PIE成熟，Wnt响应细胞及其后代丢失。虽然组织学上相似，但PIE缺乏Wnt反应性干细胞龛，并表现出慢性炎症组织的特征，这导致其与天然JE相比的次优屏障功能。
To evaluate the similarities and differences in barrier function of a peri-implant epithelium (PIE) versus a native junctional epithelium (JE). A mouse model was used wherein titanium implants were placed sub-occlusally in healed extraction sites. The PIE was examined at multiple timepoints after implant placement, to capture and understand the temporal nature of its assembly and homeostatic status. Mitotic activity, hemidesmosomal attachment apparatus, and inflammatory responses in the PIE were compared against a JE. Additionally, we evaluated whether the PIE developed a Wnt-responsive stem cell niche like a JE. The PIE developed from oral epithelium (OE) that had, by the time of implant placement, lost all characteristics of a JE. Compared with a JE, an established PIE had more proliferating cells, exhibited lower expression of attachment proteins, and had significantly more inflammatory cells in the underlying connective tissue. Wnt-responsive cells in the OE contributed to an initial PIE, but Wnt-responsive cells and their descendants were lost as the PIE matured. Although histologically similar, the PIE lacked a Wnt-responsive stem cell niche and exhibited characteristics of a chronically inflamed tissue, which contributed to its suboptimal barrier functions compared with a native JE.
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影响因子：
4.1
作者：
Mouraret S;Hunter DJ;Bardet C;Brunski JB;Bouchard P;Helms JA
通讯作者：
Helms JA
影响因子：
4.5
作者：
Kiraly O;Gong G;Olipitz W;Muthupalani S;Engelward BP
通讯作者：
Engelward BP
影响因子：
4.3
作者：
Coyac, Benjamin R.;Salvi, Giuseppe;Helms, Jill A.
通讯作者：
Helms, Jill A.
影响因子：
4.3
作者：
Ramanauskaite, Ausra;Becker, Kathrin;Schwarz, Frank
通讯作者：
Schwarz, Frank
影响因子：
4.3
作者：
BRAGA, AM;SQUIER, CA
通讯作者：
SQUIER, CA